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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1994-2-24
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pubmed:abstractText |
N-[2-(p-bromocinnamylmethylamino) ethyl]-5-isoquinolinesulfonamide (H-87), a newly synthesized inhibitor of protein kinase A, significantly decreased the drug resistance of multidrug resistant human U937/M cells, mouse FM3A/M and P388/M cells. Northern blot analysis showed MDR1 gene expression was decreased in H-87-treated P388 M cells. H-87 inhibited the activity of MDR1 (multidrug resistance-1) promoter in a dose-dependent manner in transient expression assay. In contrast, the expression of c-raf-1 gene significantly enhanced the activity of MDR1 promoter. We therefore examined the effect of H-87 on MDR1 gene expression in c-raf-1 transfected CV-1 cells (CV-1/raf). A significant decrease in the level of MDR1 mRNA was observed after H-87 treatment of the CV-1/raf cells. These results suggest that inhibition of MDR1 gene expression by H-87 is associated with circumvention of drug resistance in MDR cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Daunorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-(4-bromocinnamylamino)ethyl)-5-...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Vinblastine,
http://linkedlifedata.com/resource/pubmed/chemical/Vincristine
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
74
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pubmed:geneSymbol |
c-raf-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-41
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8287370-Animals,
pubmed-meshheading:8287370-Antineoplastic Agents,
pubmed-meshheading:8287370-Blotting, Northern,
pubmed-meshheading:8287370-Cells, Cultured,
pubmed-meshheading:8287370-Cercopithecus aethiops,
pubmed-meshheading:8287370-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8287370-Colchicine,
pubmed-meshheading:8287370-Cricetinae,
pubmed-meshheading:8287370-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:8287370-Daunorubicin,
pubmed-meshheading:8287370-Dose-Response Relationship, Drug,
pubmed-meshheading:8287370-Down-Regulation,
pubmed-meshheading:8287370-Doxorubicin,
pubmed-meshheading:8287370-Drug Resistance,
pubmed-meshheading:8287370-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8287370-Humans,
pubmed-meshheading:8287370-Isoquinolines,
pubmed-meshheading:8287370-Mesocricetus,
pubmed-meshheading:8287370-Mice,
pubmed-meshheading:8287370-Promoter Regions, Genetic,
pubmed-meshheading:8287370-Protein Kinase Inhibitors,
pubmed-meshheading:8287370-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8287370-Sulfonamides,
pubmed-meshheading:8287370-Transfection,
pubmed-meshheading:8287370-Tumor Cells, Cultured,
pubmed-meshheading:8287370-Vinblastine,
pubmed-meshheading:8287370-Vincristine
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pubmed:year |
1993
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pubmed:articleTitle |
Inhibition of MDR1 gene expression by H-87, a selective inhibitor of cAMP-dependent protein kinase.
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pubmed:affiliation |
Department of Biochemistry, College of Medicine, Pusan National University, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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