Linked Life Data

8286392

Source:http://linkedlifedata.com/resource/pubmed/id/8286392

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-2-18
pubmed:abstractText
We introduced eight bases, including four base analogs, into 15-mer triplex-forming oligonucleotides (TFOs) [d-psTTTCTTTNTTTTCTT; ps = thiophosphate; N = A, G, C, T, 2'-deoxyinosine (I), 2'-deoxyxanthosine (X), 5-methyl-2'-deoxycytidine (m5C), or 5-bromo-2'-deoxyuridine(br5U)] to investigate the Hoogsteen-like hydrogen bonding to the base in the target 34-mer strand (d-TGAGTGAGTAAAGAAARAAAAGAATGAGTGCCAA.d-TTGGCACTCATTCTTTTYTTTCT TTACTCACTCA; RY = AT, GC, TA, or CG). We examined the thermal stability of 15-mer triplexes in buffer containing 100 mM sodium acetate and 1 M NaCl at pH 5.0. The triplexes with typical triplets of T.AT (51.3 degrees C), br5U.AT (52.4 degrees C), C+.GC (66.7 degrees C), and m5C+.GC (66.8 degrees C) at the central position showed relatively higher Tm values, as expected. The relatively high stability of the X.AT triplex (39.8 degrees C) was observed. Among the N.TA triplets, G.TA (44.8 degrees C) was thermally the most stable, and moreover, the data showed that the N.TA triplet was also stabilized by I in the N position (40.7 degrees C). Furthermore, the TFOs were converted to DNA-cleaving molecules by introducing a newly synthesized 1,10-phenanthroline (OP) derivative on the thiophosphate group at the 5' end. Cleavage reactions of the 32P-labeled DNA (34-mer) were carried out. The cleavage efficiencies were compared to the Tm values of triplexes with or without an OP derivative. Results showed that the increased cleavage yields reflect the higher thermal stability of the triplex formed in most cases, but a few exceptional cases existed. Especially, the G-containing TFO did not show the above correlation between thermal stability and cleavage yield.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
606-13
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8286392-3-Mercaptopropionic Acid, pubmed-meshheading:8286392-Ascorbic Acid, pubmed-meshheading:8286392-Base Sequence, pubmed-meshheading:8286392-Binding Sites, pubmed-meshheading:8286392-Chelating Agents, pubmed-meshheading:8286392-Copper, pubmed-meshheading:8286392-Cysteine, pubmed-meshheading:8286392-DNA, pubmed-meshheading:8286392-Dithiothreitol, pubmed-meshheading:8286392-Drug Stability, pubmed-meshheading:8286392-Glutathione, pubmed-meshheading:8286392-Hot Temperature, pubmed-meshheading:8286392-Hydrogen Bonding, pubmed-meshheading:8286392-Molecular Sequence Data, pubmed-meshheading:8286392-Molecular Structure, pubmed-meshheading:8286392-Nucleic Acid Conformation, pubmed-meshheading:8286392-Nucleic Acid Denaturation, pubmed-meshheading:8286392-Oligodeoxyribonucleotides, pubmed-meshheading:8286392-Phenanthrolines
pubmed:year
1994
pubmed:articleTitle
Detailed study of sequence-specific DNA cleavage of triplex-forming oligonucleotides linked to 1,10-phenanthroline.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't