Linked Life Data

8284512

Source:http://linkedlifedata.com/resource/pubmed/id/8284512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-2-15
pubmed:abstractText
Two types of full-length cDNA clones have been constructed corresponding to the entire genome of turnip yellow mosaic virus (TYMV), from which infectious transcripts devoid of 5' non-viral extensions can be synthesized in vitro. The first type of transcript (tTYFL7) harbours 75 non-viral nucleotides at its 3' end, whereas the second type (tTYFL84) possesses only 2 non-viral nucleotides at its 3' end. The 2 kilobase-long 3' region of tTYFL84 derives from amplification by the polymerase chain reaction of the corresponding TYMV cDNA. Both tTYFL7 and tTYFL84 are infectious in rapeseed protoplasts and plants. tTYFL7 is far less infectious than wild-type TYMV RNA and somewhat less infectious than tTYFL84. The possible effects of the 3' extraviral sequences of tTYFL7 and the heterogeneity observed in the infectivity of other transcripts prepared as was tTYFL84 are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0923-2516
pubmed:author
pubmed:issnType
Print
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
In vitro transcripts of turnip yellow mosaic virus encompassing a long 3' extension or produced from a full-length cDNA clone harbouring a 2 kb-long PCR-amplified segment are infectious.
pubmed:affiliation
Institut Jacques Monod, Paris.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't