Linked Life Data

8282348

Source:http://linkedlifedata.com/resource/pubmed/id/8282348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Suppl
pubmed:dateCreated
1994-2-16
pubmed:abstractText
A dysfunctioning of Ca2+ pump ATPase in the sarcoplasmic reticulum in vascular smooth muscle has been proposed as a contributing factor for the development of genetic hypertension. In this study, we determined whether in vitro inhibition of the sarcoplasmic reticulum Ca2+ pump in vascular smooth muscle tissues and cultured cells isolated from aortas of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats would elicit the known alterations of contractile function and cell growth. We found the following common vascular effects of thapsigargin and cyclopiazonic acid, which are known to be selective inhibitors of sarcoplasmic reticulum Ca(2+)-ATPase in a number of tissues including smooth muscle: (1) Both sarcoplasmic reticulum Ca2+ pump inhibitors diminished agonist-induced transient contraction in Ca(2+)-free medium (ie, contraction due to intracellular release of Ca2+) and enhanced nifedipine-sensitive contraction on readmission of Ca2+ (ie, Ca2+ influx via L-type channels); and (2) thapsigargin and cyclopiazonic acid inhibited the attachment of cultured aortic muscle cells to the substrate in a similar degree in both SHR and WKY cells, but SHR cells were more sensitive than WKY cells to the inhibition of cell proliferation by these two agents. The first effect may provide an explanation for several contractile abnormalities known to be associated with elevated cytosolic Ca2+ concentration, whereas the second effect suggests that elevation of cytosolic Ca2+ in aortic smooth muscle cells is not necessarily associated with or sufficient to account for the accelerated cellular proliferation in SHR. These results, however, further stress the functional importance of impairment of Ca2+ regulation in vascular smooth muscle cells in genetic hypertension.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0194-911X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
I156-60
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:8282348-Animals, pubmed-meshheading:8282348-Aorta, Thoracic, pubmed-meshheading:8282348-Calcium-Transporting ATPases, pubmed-meshheading:8282348-Cell Division, pubmed-meshheading:8282348-Cells, Cultured, pubmed-meshheading:8282348-Endothelium, Vascular, pubmed-meshheading:8282348-Indoles, pubmed-meshheading:8282348-Male, pubmed-meshheading:8282348-Muscle, Smooth, Vascular, pubmed-meshheading:8282348-Muscle Contraction, pubmed-meshheading:8282348-Mycotoxins, pubmed-meshheading:8282348-Phenylephrine, pubmed-meshheading:8282348-Potassium Chloride, pubmed-meshheading:8282348-Rats, pubmed-meshheading:8282348-Rats, Inbred SHR, pubmed-meshheading:8282348-Rats, Inbred WKY, pubmed-meshheading:8282348-Rats, Wistar, pubmed-meshheading:8282348-Sarcoplasmic Reticulum, pubmed-meshheading:8282348-Species Specificity, pubmed-meshheading:8282348-Terpenes, pubmed-meshheading:8282348-Thapsigargin, pubmed-meshheading:8282348-Time Factors
pubmed:year
1994
pubmed:articleTitle
Effects of sarcoplasmic reticulum calcium pump inhibitors on vascular smooth muscle.
pubmed:affiliation
Department of Physiology, University of Hong Kong.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't