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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1994-2-8
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pubmed:abstractText |
RNA 2 of beet necrotic yellow vein virus carries the cistron for the 21 kd coat protein at its 5'-extremity. During translation, the coat protein cistron termination codon is suppressed about 10% of the time so that translation continues into the adjacent open reading frame to produce a 75 kd species, known as P75, which contains the coat protein sequence at its N-terminus. Immunoblotting experiments with a P75-specific antiserum showed that P75 is present in only trace amounts in purified virus preparations. Electron microscopic visualization of immunogold-labelled virions in crude tissue extracts has provided evidence for an association between P75 and at least a fraction of the BNYVV particles, with P75 being predominantly located near one end of the rod-shaped virions. This finding is discussed in the context of the current model for the role of P75 in virus assembly and vector transmission.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0304-8608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
195-203
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:8279955-Capsid,
pubmed-meshheading:8279955-Immunohistochemistry,
pubmed-meshheading:8279955-Microscopy, Immunoelectron,
pubmed-meshheading:8279955-Myxomycetes,
pubmed-meshheading:8279955-Plant Viruses,
pubmed-meshheading:8279955-RNA Viruses,
pubmed-meshheading:8279955-Virion,
pubmed-meshheading:8279955-Virus Replication
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pubmed:year |
1994
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pubmed:articleTitle |
Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles.
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pubmed:affiliation |
INRA, Laboratorie de Pathologie Végétale, Colmar, France.
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pubmed:publicationType |
Journal Article
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