Linked Life Data

8277358

Source:http://linkedlifedata.com/resource/pubmed/id/8277358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-2-10
pubmed:abstractText
A convenient--simple, sensitive, rapid and reproducible--enzyme immunoassay to measure H-2 particulated and solubilized cellular antigens is described. Cellular antigens were measured by ELISA through the binding of specific biotinylated antibodies and streptoavidin-peroxidase conjugate to cells in suspension. Endogenous peroxidase activity of activated cells was inhibited by addition of sodium azide and H2O2 in acid conditions. The assay proved capable of distinguishing between two cell lines (EL-4/H-2b and P815/H-2d) and even between the cells of three congenic mouse strains (BALB/B, H-2b, BALB/c, H-2d, and BALB/K, H-2k) and was sensitive to as few as 2.5 x 10(4) cells/well. Results were comparable to those obtained with FACS. An inhibition version of this assay was found to be very useful for the detection of H-2 antigens present in whole antigen cells extracts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0887-8013
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
348-52
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Immunoenzymatic assay that measures the expression of murine histocompatibility antigens in macrophages and lymphocytes.
pubmed:affiliation
Instituto de Investigaciones Biomédicas, UNAM, Mexico, D.F.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't