Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-2-10
|
| pubmed:abstractText |
Proven isogenic capsule-negative derivatives (CP9.29, CP9.108, CP9.137, CP9.171, CP9.443, and CP9.C56), generated from an O4/K54/H5 blood isolate (CP9) of Escherichia coli by IS50L::phoA (TnphoA)-mediated transposon mutagenesis, were used to assess the function of a non-K1 capsule in three animal models. Intraperitoneal injection of CP9 (K54+) into mice resulted in an LD50 at 24 h of 5.5 x 10(6) cfu compared with LD50s of 2.6 x 10(7) cfu and 3.8 x 10(7) cfu for CP9.108 (K54-) and CP9.C56 (K54-) (P < .001). CP9 was cleared less rapidly from the bloodstream, after intravascular injection, than was CP9.108 (P < .01). In the rat granuloma pouch model, CP9 could proliferate from starting inocula as low as 1.0 x 10(3) cfu/mL. In contrast, capsule-deficient derivatives underwent transient log kills with starting inocula as high as 1.0 x 10(6) cfu/mL. Because proven isogenic strains were evaluated, a clear contribution of the K54 capsular polysaccharide to virulence in vivo is demonstrated.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-1899
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
169
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
112-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8277173-Animals,
pubmed-meshheading:8277173-Antigens, Bacterial,
pubmed-meshheading:8277173-Antigens, Surface,
pubmed-meshheading:8277173-DNA Transposable Elements,
pubmed-meshheading:8277173-Disease Models, Animal,
pubmed-meshheading:8277173-Escherichia coli,
pubmed-meshheading:8277173-Escherichia coli Infections,
pubmed-meshheading:8277173-Female,
pubmed-meshheading:8277173-Humans,
pubmed-meshheading:8277173-Immunoelectrophoresis,
pubmed-meshheading:8277173-Lethal Dose 50,
pubmed-meshheading:8277173-Liver,
pubmed-meshheading:8277173-Male,
pubmed-meshheading:8277173-Metabolic Clearance Rate,
pubmed-meshheading:8277173-Mice,
pubmed-meshheading:8277173-Mice, Inbred ICR,
pubmed-meshheading:8277173-Mutagenesis, Insertional,
pubmed-meshheading:8277173-Rats,
pubmed-meshheading:8277173-Rats, Wistar,
pubmed-meshheading:8277173-Spleen,
pubmed-meshheading:8277173-Time Factors,
pubmed-meshheading:8277173-Virulence
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
The presence of K54 capsular polysaccharide increases the pathogenicity of Escherichia coli in vivo.
|
| pubmed:affiliation |
Bacterial Pathogenesis Unit, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892.
|
| pubmed:publicationType |
Journal Article
|