| pubmed-article:8276107 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8276107 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:8276107 | lifeskim:mentions | umls-concept:C0376180 | lld:lifeskim |
| pubmed-article:8276107 | lifeskim:mentions | umls-concept:C2248769 | lld:lifeskim |
| pubmed-article:8276107 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:8276107 | lifeskim:mentions | umls-concept:C0054889 | lld:lifeskim |
| pubmed-article:8276107 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8276107 | pubmed:dateCreated | 1994-2-4 | lld:pubmed |
| pubmed-article:8276107 | pubmed:abstractText | We made a mutated progastrin cDNA construct that contains a cleavage site (-Arg(-4)-Arg(-3)-Lys(-2)-Arg-1) specific for the Kex2-like endoprotease furin, located ahead of the bioactive gastrin. For expressing the mutated progastrin cDNA, we used two non-endocrine cell lines, CHO and COS-7. CHO cells exhibit amidating enzyme activity and levels of amidation enzyme mRNA as high as those in the pituitary-derived endocrine cell line GH3, whereas COS-7 cells have far less amidating activity and lower amounts of mRNA. Mutant progastrin-expressing CHO cells produced mostly amidated gastrin. Gel filtration showed the size of this gastrin corresponded to that of the synthetic human gastrin-17. In contrast, COS-7 cells produced glycine-extended gastrin and only a small amount of amidated gastrin. The difference in the amount of amidated gastrin products produced by the two non-endocrine cell lines is due to differing amounts of the amidation enzyme contained in each cell line. | lld:pubmed |
| pubmed-article:8276107 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8276107 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8276107 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8276107 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8276107 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:8276107 | pubmed:author | pubmed-author:TakeuchiTT | lld:pubmed |
| pubmed-article:8276107 | pubmed:author | pubmed-author:HayashiNN | lld:pubmed |
| pubmed-article:8276107 | pubmed:author | pubmed-author:SuganoKK | lld:pubmed |
| pubmed-article:8276107 | pubmed:author | pubmed-author:KayeZZ | lld:pubmed |
| pubmed-article:8276107 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8276107 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:8276107 | pubmed:volume | 337 | lld:pubmed |
| pubmed-article:8276107 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8276107 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8276107 | pubmed:pagination | 27-32 | lld:pubmed |
| pubmed-article:8276107 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8276107 | pubmed:meshHeading | pubmed-meshheading:8276107-... | lld:pubmed |
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| pubmed-article:8276107 | pubmed:meshHeading | pubmed-meshheading:8276107-... | lld:pubmed |
| pubmed-article:8276107 | pubmed:meshHeading | pubmed-meshheading:8276107-... | lld:pubmed |
| pubmed-article:8276107 | pubmed:meshHeading | pubmed-meshheading:8276107-... | lld:pubmed |
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| pubmed-article:8276107 | pubmed:meshHeading | pubmed-meshheading:8276107-... | lld:pubmed |
| pubmed-article:8276107 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8276107 | pubmed:articleTitle | Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7. | lld:pubmed |
| pubmed-article:8276107 | pubmed:affiliation | Division of Molecular Endocrinology, Gunma University, Maebashi, Japan. | lld:pubmed |
| pubmed-article:8276107 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8276107 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8276107 | lld:pubmed |
