Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-2-4
pubmed:abstractText
We made a mutated progastrin cDNA construct that contains a cleavage site (-Arg(-4)-Arg(-3)-Lys(-2)-Arg-1) specific for the Kex2-like endoprotease furin, located ahead of the bioactive gastrin. For expressing the mutated progastrin cDNA, we used two non-endocrine cell lines, CHO and COS-7. CHO cells exhibit amidating enzyme activity and levels of amidation enzyme mRNA as high as those in the pituitary-derived endocrine cell line GH3, whereas COS-7 cells have far less amidating activity and lower amounts of mRNA. Mutant progastrin-expressing CHO cells produced mostly amidated gastrin. Gel filtration showed the size of this gastrin corresponded to that of the synthetic human gastrin-17. In contrast, COS-7 cells produced glycine-extended gastrin and only a small amount of amidated gastrin. The difference in the amount of amidated gastrin products produced by the two non-endocrine cell lines is due to differing amounts of the amidation enzyme contained in each cell line.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
337
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8276107-Amino Acid Sequence, pubmed-meshheading:8276107-Animals, pubmed-meshheading:8276107-Binding Sites, pubmed-meshheading:8276107-CHO Cells, pubmed-meshheading:8276107-Cell Line, pubmed-meshheading:8276107-Cercopithecus aethiops, pubmed-meshheading:8276107-Chromatography, Gel, pubmed-meshheading:8276107-Cricetinae, pubmed-meshheading:8276107-DNA, Complementary, pubmed-meshheading:8276107-Furin, pubmed-meshheading:8276107-Gastrins, pubmed-meshheading:8276107-Gene Expression, pubmed-meshheading:8276107-Humans, pubmed-meshheading:8276107-Kidney, pubmed-meshheading:8276107-Mixed Function Oxygenases, pubmed-meshheading:8276107-Molecular Sequence Data, pubmed-meshheading:8276107-Multienzyme Complexes, pubmed-meshheading:8276107-Mutagenesis, pubmed-meshheading:8276107-Protein Precursors, pubmed-meshheading:8276107-RNA, Messenger, pubmed-meshheading:8276107-Rats, pubmed-meshheading:8276107-Subtilisins, pubmed-meshheading:8276107-Transfection
pubmed:year
1994
pubmed:articleTitle
Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7.
pubmed:affiliation
Division of Molecular Endocrinology, Gunma University, Maebashi, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't