Linked Life Data

8274535

Source:http://linkedlifedata.com/resource/pubmed/id/8274535

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1994-2-7
pubmed:abstractText
The metabolism of 2-deoxy-D-glucose (DG) in glucose grown repressed Saccharomyces cerevisiae cells was studied in the absence and presence of glucose (Glc) at 30 degrees C in a standard pyrophosphate medium containing 4.5 x 10(7) cells/ml. 1H- and 13C-NMR spectroscopy were successfully used to distinguish and identify several derivatives of DG and trehalose. Using [1-13C]DG, alpha- and beta-DG, alpha- and beta-DG6P, dideoxy-trehalose (DG-DG) and deoxy-trehalose (DG-Glc) can be simultaneously observed in the intracellular medium. The [DG6P]/[DG] ratio is about 5-6. The results seem to indicate the existence of an equilibrium between DG and DG6P, which limits the production of DG6P in cells. Glucose was found to exert a great influence on the metabolism of DG. It favours the formation of DG-DG and DG-Glc.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-9084
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
825-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Influence of glucose on the deoxyglucose metabolism in S cerevisiae: detection and identification of deoxyglucose and trehalose derivatives by 1H- and 13C-NMR spectroscopy.
pubmed:affiliation
Département de Biologie Cellulaire et Moléculaire, CEN Saclay, Gif-sur-Yvette, France.
pubmed:publicationType
Journal Article, Comparative Study