Linked Life Data

8274520

Source:http://linkedlifedata.com/resource/pubmed/id/8274520

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-2-4
pubmed:abstractText
Trypsin was modified with various vinyl polymers by the graft polymerization of vinyl monomers using a trypsin derivative containing aliphatic azo groups as the initiator. The graft polymers were chosen to be sensitive to external signals, e.g., a redox-sensitive poly[3-carbamoyl-1-(p-vinylbenzyl)pyridinium chloride] and a pH-sensitive poly(methacrylic acid-co-methyl methacrylate). The trypsin modified with the redox-sensitive polymer became insoluble in water in the presence of Na2S2O4, and redissolved when H2O2 was added. However, the enzymatic activity of the homogeneous solution was not proportional to the concentration of the modified trypsin remaining in the solution. The trypsin modified with the pH-sensitive polymer was insoluble and soluble in acidic and neutral solutions, respectively. The enzymatic activity of the homogeneous solution changed reversibly with pH in proportion to the concentration of the modified trypsin remaining in the solution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1043-1802
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
358-61
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:articleTitle
Trypsin modification by vinyl polymers with variable solubilities in response to external signals.
pubmed:affiliation
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
pubmed:publicationType
Journal Article