Linked Life Data

8274151

Source:http://linkedlifedata.com/resource/pubmed/id/8274151

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-1-31
pubmed:abstractText
Fatty acids have recently been demonstrated to activate peroxisome proliferator-activated receptors (PPARs) but specific structural requirements of fatty acids to produce this response have not yet been determined. Importantly, it has hitherto not been possible to show specific binding of these compounds to PPAR. To test whether a common PPAR binding metabolite might be formed, we tested the effects of long-chain omega-3 polyunsaturated fatty acids, differentially beta-oxidizable fatty acids and inhibitors of fatty acid metabolism. We determined the activation of a reporter gene by a chimaeric receptor encompassing the DNA binding domain of the glucocorticoid receptor and the ligand binding domain of PPAR. The omega-3 unsaturated fatty acids were slightly more potent PPAR activators in vitro than saturated fatty acids. The peroxisomal proliferation-inducing, non-beta-oxidizable, tetradecylthioacetic acid activated PPAR to the same extent as the strong peroxisomal proliferator WY 14,643, whereas the homologous beta-oxidizable tetradecylthiopropionic acid was only as potent as a non-substituted fatty acid. Cyclooxygenase inhibitors, radical scavengers or cytochrome P450 inhibitors did not affect activation of PPAR. In conclusion, beta-oxidation is apparently not required for the formation of the PPAR-activating molecule and this moiety might be a fatty acid, its ester with CoA, or a further derivative of the activated fatty acid prior to beta-oxidation of the acyl-CoA ester. These data should aid understanding of signal transduction via PPAR and the identification of a receptor ligand.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2177-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8274151-Animals, pubmed-meshheading:8274151-Chimera, pubmed-meshheading:8274151-Cyclooxygenase Inhibitors, pubmed-meshheading:8274151-Cytochrome P-450 Enzyme System, pubmed-meshheading:8274151-Fatty Acids, pubmed-meshheading:8274151-Fatty Acids, Omega-3, pubmed-meshheading:8274151-Free Radical Scavengers, pubmed-meshheading:8274151-Liver, pubmed-meshheading:8274151-Male, pubmed-meshheading:8274151-Microbodies, pubmed-meshheading:8274151-Oxidation-Reduction, pubmed-meshheading:8274151-Rats, pubmed-meshheading:8274151-Rats, Wistar, pubmed-meshheading:8274151-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:8274151-Receptors, Glucocorticoid, pubmed-meshheading:8274151-Serum Albumin, Bovine, pubmed-meshheading:8274151-Sulfur, pubmed-meshheading:8274151-Transcription Factors
pubmed:year
1993
pubmed:articleTitle
Structural and metabolic requirements for activators of the peroxisome proliferator-activated receptor.
pubmed:affiliation
Department of Medical Nutrition, Karolinska Institute, Huddinge Hospital, NOVUM, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't