|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0178487,
umls-concept:C0303611,
umls-concept:C0439851,
umls-concept:C1167624,
umls-concept:C1261322,
umls-concept:C1548779,
umls-concept:C1552596,
umls-concept:C1947902,
umls-concept:C1947931,
umls-concept:C2827499
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pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-2-3
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|
pubmed:abstractText |
Recently, a method for the direct labeling of antibodies with 99mTc was described in which sulfhydryls were reportedly generated by reduction of antibody disulfides with ascorbic acid. Thereafter, these proteins may be labeled at high efficiency with 99mTc following reduction of pertechnetate with dithionite. This investigation was initially conducted to evaluate the mechanism of the increased stability towards cysteine challenge reported for the label and subsequently to determine the role of ascorbate in the labeling process.
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pubmed:grant |
|
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pubmed:commentsCorrections |
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pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0161-5505
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
35
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
127-34
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|
pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
|
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pubmed:year |
1994
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|
pubmed:articleTitle |
Investigations of ascorbate for direct labeling of antibodies with technetium-99m.
|
|
pubmed:affiliation |
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
