Linked Life Data

8269059

Source:http://linkedlifedata.com/resource/pubmed/id/8269059

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-2-2
pubmed:abstractText
The effect of the addition of cetyltrimethylammonium bromide (CTAB) to the buffer system in capillary electrophoresis on electroosmotic flow (EOF) is examined. At a CTAB concentration of 2.5 x 10(-4) M, EOF is anodal (flow towards the positive detector column end). With bare silica columns, anodal EOF first increases with increasing pH, up to a maximum in the pH range 4-6 depending on CTAB concentration, then decreases as pH is further increased. Optimum resolution of pyridinecarboxylic acid isomers is obtained at pH 2.7 with a 10 mM phosphate buffer and 30 mM CTAB. Using the same buffer system, optimum resolution for hydroxy-substituted pyridinecarboxylic acid isomers is obtained at pH 7.5. The use of CTAB results in a dramatic improvement in peak shape. Preliminary results, using an excimer laser operated at 248 nm, show that the fluorescence intensity of isonicotinic acid is substantially enhanced with the addition of 0.3% hydrogen peroxide to the phosphate buffer system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
653
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-7
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Separation of pyridinecarboxylic acid isomers and related compounds by capillary zone electrophoresis. Effect of cetyltrimethylammonium bromide on electroosmotic flow and resolution.
pubmed:affiliation
Program Resources, Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD 21702.
pubmed:publicationType
Journal Article