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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-1-25
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pubmed:abstractText |
Two membrane bound enzymes which tautomerize L-dopachrome and are specific for the L-isomer of dopachrome have been defined in melanin forming cells. Another enzyme that tautomerizes D-dopachrome with concomitant decarboxylation to give 5,6-dihydroxyindole (DHI) was found in the cytoplasm of human melanoma cells, human liver and in all of the organs studied in rat. The decolorization of D-dopachrome with the formation of DHI was used in monitoring the isolation of a tautomerase from liver of male rats and therefore the enzyme is provisionally called D-dopachrome tautomerase. The molecular weight of D-dopachrome tautomerase monomer was approximately 12 kD and its N-terminal amino acid sequence was P-F-V-E-L-E-T-N-L-P-A-. The Km for D-dopachrome was 1.5 mM and Vmax 0.5 mmol per min and mg protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
197
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
619-24
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8267597-Amino Acid Sequence,
pubmed-meshheading:8267597-Animals,
pubmed-meshheading:8267597-Brain,
pubmed-meshheading:8267597-Chromatography, Gel,
pubmed-meshheading:8267597-Chromatography, High Pressure Liquid,
pubmed-meshheading:8267597-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8267597-Humans,
pubmed-meshheading:8267597-Indolequinones,
pubmed-meshheading:8267597-Indoles,
pubmed-meshheading:8267597-Intramolecular Oxidoreductases,
pubmed-meshheading:8267597-Isomerases,
pubmed-meshheading:8267597-Kidney,
pubmed-meshheading:8267597-Kinetics,
pubmed-meshheading:8267597-Liver,
pubmed-meshheading:8267597-Male,
pubmed-meshheading:8267597-Melanoma,
pubmed-meshheading:8267597-Molecular Sequence Data,
pubmed-meshheading:8267597-Molecular Weight,
pubmed-meshheading:8267597-Myocardium,
pubmed-meshheading:8267597-Peptide Fragments,
pubmed-meshheading:8267597-Quinones,
pubmed-meshheading:8267597-Rats,
pubmed-meshheading:8267597-Rats, Sprague-Dawley,
pubmed-meshheading:8267597-Sequence Homology, Amino Acid,
pubmed-meshheading:8267597-Spleen,
pubmed-meshheading:8267597-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Isolation of a new tautomerase monitored by the conversion of D-dopachrome to 5,6-dihydroxyindole.
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pubmed:affiliation |
Department of Pharmacology, University of Lund, Sweden.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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