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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1994-1-21
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pubmed:abstractText |
Transcription of the calcitonin (CT) gene in the medullary thyroid carcinoma (MTC) cell line TT is modulated by a neuroendocrine-specific enhancer fragment (nucleotides -965 to -905) containing two CANNTG motifs (E2 and E3) and an ETs-like response element. To determine the cell-specific component of this fragment, oligonucleotides containing the individual elements were inserted in front of a minimal CT promoter and tested for reporter protein production in CT-positive (TT) and -negative (RO-D81 and HeLa) cells. In TT cells, using two copies of E2 or four copies of Ets increased minimal promoter activity a 20-40 fold. Using two copies of E3 had no effect on minimal promoter activity. In CT-negative MTC cells (RO-D81), the Ets response element was active but the two copies of E2 were not. Similar results were obtained with the non-neuroendocrine cell-line HeLa. I therefore concluded that E2 was the cell-type-specific component of the enhancer. An E2-specific binding protein was detected in both MTC cell lines but not in HeLa. This protein had different mobility and DNA-binding specificity in CT-positive TT cells and CT-negative RO-D81 cells. In conclusion, the CAGCTG motif of E2 modulated the cell-specific transcription of the CT gene, and its inactivation in CT-negative MTC cells correlated with modifications in its binding proteins.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1324418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1517207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1710033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1850695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1892659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-1987636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2035191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2044959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2157143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2200736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2280775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2503252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2538133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2897632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2898300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-2901348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-3043185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-3091258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-3112776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-3607875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-3690668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-6089345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-6798062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8265349-8232320
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
21
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pubmed:geneSymbol |
CT
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5360-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8265349-Base Sequence,
pubmed-meshheading:8265349-Calcitonin,
pubmed-meshheading:8265349-Carcinoma, Medullary,
pubmed-meshheading:8265349-Consensus Sequence,
pubmed-meshheading:8265349-DNA-Binding Proteins,
pubmed-meshheading:8265349-Enhancer Elements, Genetic,
pubmed-meshheading:8265349-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8265349-HeLa Cells,
pubmed-meshheading:8265349-Humans,
pubmed-meshheading:8265349-Molecular Sequence Data,
pubmed-meshheading:8265349-Oligodeoxyribonucleotides,
pubmed-meshheading:8265349-RNA, Messenger,
pubmed-meshheading:8265349-Thyroid Neoplasms,
pubmed-meshheading:8265349-Transcription Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine-specific CANNTG motif of the calcitonin gene.
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pubmed:affiliation |
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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