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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-1-21
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pubmed:abstractText |
The macrophage colony-stimulating factor (M-CSF) receptor is expressed in a tissue-specific fashion from two distinct promoters in monocytes/macrophages and the placenta. In order to further understand the transcription factors which play a role in the commitment of multipotential progenitors to the monocyte/macrophage lineage, we have initiated an investigation of the factors which activate the M-CSF receptor very early during the monocyte differentiation process. Here we demonstrate that the human monocytic M-CSF receptor promoter directs reporter gene activity in a tissue-specific fashion. Since one of the few transcription factors which have been implicated in the regulation of monocyte genes is the macrophage- and B-cell-specific PU.1 transcription factor, we investigated whether PU.1 binds and activates the M-CSF receptor promoter. Here we demonstrate that both in vitro-translated PU.1 and PU.1 from nuclear extracts bind to a specific site in the M-CSF receptor promoter just upstream from the major transcription initiation site. Mutations in this site which eliminate PU.1 binding decrease M-CSF receptor promoter activity significantly in macrophage cell lines only. Furthermore, PU.1 transactivates the M-CSF receptor promoter in nonmacrophage cells. These results suggest that PU.1 plays a major role in macrophage gene regulation and development by directing the expression of a receptor for a key macrophage growth factor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1339307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1346576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1373332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1406622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1531036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1620109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1655505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1689301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1693527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1701692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1722028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1729611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-1876833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2142747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2153029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2180582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2371282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2406720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2408759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2421165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2467742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2523515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2524648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2526662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2594367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2828177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2833708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2846185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2849512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2908509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2974321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-2987824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-3162233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-3273409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-3476856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-4028159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-6893015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8095266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8096519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8434021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8445950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8456286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264604-8474451
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
373-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8264604-Humans,
pubmed-meshheading:8264604-DNA,
pubmed-meshheading:8264604-Monocytes,
pubmed-meshheading:8264604-Macrophages,
pubmed-meshheading:8264604-Base Sequence,
pubmed-meshheading:8264604-Amino Acid Sequence,
pubmed-meshheading:8264604-Tissue Distribution,
pubmed-meshheading:8264604-Binding Sites,
pubmed-meshheading:8264604-Cell Line,
pubmed-meshheading:8264604-Molecular Sequence Data,
pubmed-meshheading:8264604-Transcription, Genetic,
pubmed-meshheading:8264604-Gene Expression Regulation,
pubmed-meshheading:8264604-B-Lymphocytes,
pubmed-meshheading:8264604-Promoter Regions, Genetic,
pubmed-meshheading:8264604-DNA-Binding Proteins,
pubmed-meshheading:8264604-Transcription Factors,
pubmed-meshheading:8264604-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:8264604-Mutagenesis, Site-Directed,
pubmed-meshheading:8264604-Receptor, Macrophage Colony-Stimulating Factor
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