|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0036025,
umls-concept:C0040649,
umls-concept:C0282498,
umls-concept:C1145667,
umls-concept:C1167622,
umls-concept:C1515877,
umls-concept:C1521761,
umls-concept:C1879547,
umls-concept:C2349209,
umls-concept:C2825311
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-1-21
|
|
pubmed:abstractText |
After each round of replication, new transcription initiation complexes must assemble on promoter DNA. This process may compete with packaging of the same promoter sequences into nucleosomes. To elucidate interactions between regulatory transcription factors and nucleosomes on newly replicated DNA, we asked whether heat shock factor (HSF) could be made to bind to nucleosomal DNA in vivo. A heat shock element (HSE) was embedded at either of two different sites within a DNA segment that directs the formation of a stable, positioned nucleosome. The resulting DNA segments were coupled to a reporter gene and transfected into the yeast Saccharomyces cerevisiae. Transcription from these two plasmid constructions after induction by heat shock was similar in amount to that from a control plasmid in which HSF binds to nucleosome-free DNA. High-resolution genomic footprint mapping of DNase I and micrococcal nuclease cleavage sites indicated that the HSE in these two plasmids was, nevertheless, packaged in a nucleosome. The inclusion of HSE sequences within (but relatively close to the edge of) the nucleosome did not alter the position of the nucleosome which formed with the parental DNA fragment. Genomic footprint analyses also suggested that the HSE-containing nucleosome was unchanged by the induction of transcription. Quantitative comparisons with control plasmids ruled out the possibility that HSF was bound only to a small fraction of molecules that might have escaped nucleosome assembly. Analysis of the helical orientation of HSE DNA in the nucleosome indicated that HSF contacted DNA residues that faced outward from the histone octamer. We discuss the significance of these results with regard to the role of nucleosomes in inhibiting transcription and the normal occurrence of nucleosome-free regions in promoters.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1316804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1335747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1365915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-13860347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1465613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1508199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1693332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1731219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1846668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1846670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1849080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1885667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1899357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1959130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-1986252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2034656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2065977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2087783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2155706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2170977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2175361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2216769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2275823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2279025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2405254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2405281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2590940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2686840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2689876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-2846275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-287052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3006927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3037338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3044613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3052270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3062378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3115592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3146692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3198625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3306402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3320966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3352748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3532106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3548764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3711119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3889654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-3912515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6086935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6096135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6264467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6482966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6572008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6631965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-6722879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-8422685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-8450881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-8463277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-8463341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8264586-8480368
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0270-7306
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
14
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
189-99
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
