Linked Life Data

8263968

Source:http://linkedlifedata.com/resource/pubmed/id/8263968

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-1-21
pubmed:abstractText
In a recent work we have shown that neuromodulin (Nm, also known as GAP-43), a protein kinase C substrate, previously believed to be expressed exclusively in neurons, is also present in glial cells. Here we investigated the expression of Nm and its mRNA in O-2A glial progenitor cells (common precursor for oligodendrocytes and type-2 astrocytes) during their development in secondary culture and under the influence of basic fibroblast growth factor (bFGF). The different stages of oligodendrocyte development were characterized by the expression of surface markers: A2B5, which identifies O-2A glial precursor cells, and O4 and galactocerebroside (GC), which characterize later developmental stages. The number of cells expressing Nm (about 90% at culture initiation) decreased rapidly during the first 2 days and reached a plateau at around 30-40%. The level of Nm mRNA followed a similar kinetic. Immunocytochemistry demonstrated that at 4 days in vitro about 25-30% cells were A2B5+, 30-40% Nm+, a high percentage (60-70%) O4+, and 35-40% GC+. Nearly all of the morphologically immature A2B5+ cells expressed also the Nm antigen, very few of the O4+ cells still expressed Nm and almost no cells expressed both GC and Nm. Most O4+ cells developed a typical oligodendrocyte morphology and were essentially GC+. This study also showed that in the presence of serum, the A2B5+ Nm+ and O4+ Nm+ (GC-) cells retained their bipotentiality and differentiated into GFAP+ (glial fibrillary acidic protein) Nm+ type-2 astrocytes. The bFGF was found to stimulate the proliferation of Nm+ 0-2A precursor cells and to increase the level of Nm mRNA. At 4 days under this culture condition, the predominant cell type was A2B5+ and Nm+. Only 25-35% of the cells were O4+, but 90-95% of them were Nm+. Very few GC+ cells were visible in the presence of bFGF, but 20-40% of them were Nm+. These data indicate that Nm is essentially associated to glial O-2A precursor cells and further confirm that bFGF blocks the differentiation of these cells. It is suggested that Nm plays a role in the plasticity (developmental potential) of the bipotential 0-2A progenitor cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8263968-Animals, pubmed-meshheading:8263968-Animals, Newborn, pubmed-meshheading:8263968-Blotting, Western, pubmed-meshheading:8263968-Cell Differentiation, pubmed-meshheading:8263968-Cell Nucleus, pubmed-meshheading:8263968-Cells, Cultured, pubmed-meshheading:8263968-DNA, pubmed-meshheading:8263968-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8263968-Fibroblast Growth Factor 2, pubmed-meshheading:8263968-Fluorescent Antibody Technique, pubmed-meshheading:8263968-GAP-43 Protein, pubmed-meshheading:8263968-Glial Fibrillary Acidic Protein, pubmed-meshheading:8263968-Immunohistochemistry, pubmed-meshheading:8263968-Iodine Radioisotopes, pubmed-meshheading:8263968-Membrane Glycoproteins, pubmed-meshheading:8263968-Nerve Tissue Proteins, pubmed-meshheading:8263968-Neurofilament Proteins, pubmed-meshheading:8263968-Neuroglia, pubmed-meshheading:8263968-Phosphorylation, pubmed-meshheading:8263968-RNA, Messenger, pubmed-meshheading:8263968-Rats, pubmed-meshheading:8263968-Stem Cells, pubmed-meshheading:8263968-Uridine
pubmed:year
1993
pubmed:articleTitle
Expression of neuromodulin (GAP-43) and its regulation by basic fibroblast growth factor during the differentiation of O-2A progenitor cells.
pubmed:affiliation
Laboratoire de Neurobiologie Ontogénique, CNRS UPR 417, Centre de Neurochimie, Strasbourg, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't