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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
36
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pubmed:dateCreated |
1994-1-27
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pubmed:abstractText |
S-Lac lectins are a family of soluble lactose-binding animal lectins, some of which have been implicated in modulating cell-cell and cell-matrix interactions through specific carbohydrate-mediated recognition. We report here the x-ray crystal structure of a representative member of this family, the human dimeric S-Lac lectin, L-14-II, in complex with lactose, at 2.9-A resolution. The two-fold symmetric dimer is made up of two extended anti-parallel beta-sheets, which associate in a beta-sandwich motif. Remarkably, the L-14-II monomer shares not only the same topology, but a very similar beta-sheet structure with that of the leguminous plant lectins, suggesting a conserved structure-function relationship. Carbohydrate binding by L-14-II was found to involve protein residues that are very highly conserved among all S-Lac lectins. These residues map to a single DNA exon, suggesting a carbohydrate binding cassette common to all S-Lac lectins.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
25
|
pubmed:volume |
268
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27034-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8262940-Amino Acid Sequence,
pubmed-meshheading:8262940-Binding Sites,
pubmed-meshheading:8262940-Carbohydrate Metabolism,
pubmed-meshheading:8262940-Crystallography, X-Ray,
pubmed-meshheading:8262940-Galectins,
pubmed-meshheading:8262940-Hemagglutinins,
pubmed-meshheading:8262940-Humans,
pubmed-meshheading:8262940-Hydrogen Bonding,
pubmed-meshheading:8262940-Lactose,
pubmed-meshheading:8262940-Lectins,
pubmed-meshheading:8262940-Molecular Sequence Data,
pubmed-meshheading:8262940-Protein Conformation,
pubmed-meshheading:8262940-Sequence Homology, Amino Acid
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pubmed:year |
1993
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pubmed:articleTitle |
X-ray crystal structure of the human dimeric S-Lac lectin, L-14-II, in complex with lactose at 2.9-A resolution.
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pubmed:affiliation |
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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