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rdf:type |
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lifeskim:mentions |
umls-concept:C0006104,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0081889,
umls-concept:C0441655,
umls-concept:C1171362,
umls-concept:C1514873,
umls-concept:C1515670,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
2
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pubmed:dateCreated |
1994-1-26
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pubmed:abstractText |
Calmodulin-dependent protein kinase IV (CaM-kinase IV) is a Ca(2+)-responsive multifunctional protein kinase which occurs abundantly in the brain. When cDNA for rat brain CaM-kinase IV was expressed in Escherichia coli, the enzyme was produced in a good yield, but it did not show significant activity. The inactive recombinant CaM-kinase IV was phosphorylated and became highly active on incubation with a rat brain extract in the presence of both Ca2+/calmodulin and ATP/Mg2+. The recombinant CaM-kinase IV-activating activity in brain was one to two orders of magnitude higher than that in the other tissues examined. These observations suggest that CaM-kinase IV may undergo a posttranslational modification, probably Ca2+/calmodulin-dependent phosphorylation by CaM-kinase IV kinase, before exhibiting activity in the central nervous system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-70
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:8262894-Animals,
pubmed-meshheading:8262894-Base Sequence,
pubmed-meshheading:8262894-Brain,
pubmed-meshheading:8262894-Calcium-Calmodulin-Dependent Protein Kinase Type 4,
pubmed-meshheading:8262894-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:8262894-Cloning, Molecular,
pubmed-meshheading:8262894-DNA, Complementary,
pubmed-meshheading:8262894-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8262894-Enzyme Activation,
pubmed-meshheading:8262894-Escherichia coli,
pubmed-meshheading:8262894-Female,
pubmed-meshheading:8262894-Male,
pubmed-meshheading:8262894-Molecular Sequence Data,
pubmed-meshheading:8262894-Phosphorylation,
pubmed-meshheading:8262894-Protein Processing, Post-Translational,
pubmed-meshheading:8262894-Rats,
pubmed-meshheading:8262894-Rats, Wistar,
pubmed-meshheading:8262894-Recombinant Proteins,
pubmed-meshheading:8262894-Tissue Extracts
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pubmed:year |
1993
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pubmed:articleTitle |
Requirement of brain extract for the activity of brain calmodulin-dependent protein kinase IV expressed in Escherichia coli.
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pubmed:affiliation |
Department of Biochemistry, Asahikawa Medical College, Hokkaido.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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