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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-1-21
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pubmed:abstractText |
Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0015-5632
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:geneSymbol |
thr
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8262444-Brevibacterium,
pubmed-meshheading:8262444-Escherichia coli,
pubmed-meshheading:8262444-Gene Expression,
pubmed-meshheading:8262444-Genes, Bacterial,
pubmed-meshheading:8262444-Genetic Vectors,
pubmed-meshheading:8262444-Homoserine Dehydrogenase,
pubmed-meshheading:8262444-Lysine,
pubmed-meshheading:8262444-Operon,
pubmed-meshheading:8262444-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:8262444-Plasmids,
pubmed-meshheading:8262444-Threonine
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pubmed:year |
1993
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pubmed:articleTitle |
Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli.
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pubmed:affiliation |
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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