Linked Life Data

8262241

Source:http://linkedlifedata.com/resource/pubmed/id/8262241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-1-27
pubmed:abstractText
A large component of the whole-cell currents through Ca2+ channels in bovine adrenomedullary chromaffin cells has been shown to be insensitive to both L-type and N-type Ca2+ channel blockers, suggesting the existence of a third type of Ca2+ channel. In the present paper, omega-agatoxin-IVA (AgTx), a selective blocker of P-type Ca2+ channels in mammalian neurons, has been used to investigate the presence of this subtype of Ca2+ channel in bovine chromaffin cells. Barium currents (IBa) through Ca2+ channels were recorded in whole-cell patch-clamped bovine chromaffin cells. IBa was blocked by AgTx in a dose-dependent and irreversible manner. At the maximal concentration used (1 microM), AgTx inhibited IBa by 49.5 +/- 3%. Such a blockade was also present when bovine chromaffin cells were pretreated with 10 microM furnidipine, a novel 1,4-dihydropyridine L-type channel blocker, and after treatment with 1 microM of the N-type channel blocker, omega-conotoxin GVIA (CgTx). A combination of these three types of Ca2+ channel blockers suppressed the macroscopic Ba2+ currents by 88%. We conclude that bovine chromaffin cells, in addition to N- and L-type Ca2+ channels, possess a P-like component in their whole-cell currents through the Ca2+ channels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
336
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
259-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
omega-Agatoxin-IVA-sensitive calcium channels in bovine chromaffin cells.
pubmed:affiliation
Departamento de Farmacología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't