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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1994-1-19
|
| pubmed:abstractText |
A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0736-0118
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
49-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8258994-Animals,
pubmed-meshheading:8258994-Antibodies, Monoclonal,
pubmed-meshheading:8258994-Antigens, Surface,
pubmed-meshheading:8258994-Biotin,
pubmed-meshheading:8258994-Humans,
pubmed-meshheading:8258994-Immunophenotyping,
pubmed-meshheading:8258994-Lymphocyte Subsets,
pubmed-meshheading:8258994-Mice,
pubmed-meshheading:8258994-Receptors, Cell Surface,
pubmed-meshheading:8258994-Rosette Formation
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Separation of rare cell subpopulations with the aid of biotin-labelled ligands.
|
| pubmed:affiliation |
Department of Pathology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|