Linked Life Data

8258363

Source:http://linkedlifedata.com/resource/pubmed/id/8258363

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-1-19
pubmed:abstractText
We investigated the effect of melittin and Ca2+ ionophore A23187 on protein tyrosine phosphatase activity in HER14 cells (NIH-3T3 cells transfected with human epidermal growth factor 'EGF' receptor). Cell fractions were used to measure protein tyrosine phosphatase activity in vitro using 32P-labeled poly(Glu/Tyr) (4:1) peptide as a substrate. Treatment of HER14 cells with melittin or with A23187, inhibited protein tyrosine phosphatase activity in the cell sonicate and homogenate, as well as in cytosolic and particulate fractions of these cells. The inhibitory effect of both drugs was prevented by preincubating cells with EGTA (ethyleneglycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid). The cyclooxygenase inhibitor indomethacin enhanced the inhibitory effect of A23187, but not that of melittin. Addition of arachidonic acid to the cells partially prevented the inhibition of protein tyrosine phosphatase activity by melittin or A23187. Preexposure of cells to EGF enhanced the inhibitory effect of melittin--but not that of A23187. Addition of CaCl2, or MgCl2 to the cell homogenate inhibited protein tyrosine phosphatase activity. These results show that protein tyrosine phosphatase activity in HER14 cells is inhibited by melittin and Ca2+ ionophore A23187 through a Ca(2+)-dependent mechanism, and is regulated by arachidonic acid metabolism and EGF receptor activation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
247
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Inhibition of protein tyrosine phosphatase activity in HER14 cells by melittin and Ca2+ ionophore A23187.
pubmed:affiliation
Department of Pharmacology, New York University Medical Center, NY 10016.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.