|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
49
|
|
pubmed:dateCreated |
1994-1-19
|
|
pubmed:abstractText |
13C-NMR techniques in oriented bilayer systems composed of DMPC and CHAPSO have been used to measure dipolar interactions between 13C-13C and 13C-1H pairs located in the sialic acid moiety of ganglioside GM3. These interactions are reduced to a structural and motional model for the headgroup of this glycolipid using an order matrix approach. The analysis shows an average structure possessing a high degree of order and is most consistent with structures that are well extended from the membrane surface. Saturation of the ganglioside environment with high concentrations of Ca2+ (0.28 M) produces a small perturbation of the headgroup. GM3 dissolved in a DMPC/CHAPSO system can also be demonstrated to bind wheat germ agglutinin (WGA). The minimal perturbation of structure-dependent parameters suggests that the dominant structure of the sialic acid moiety in the isolated membrane system is favored for binding by the protein.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0006-2960
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
32
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
13405-13
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8257677-Chemistry, Physical,
pubmed-meshheading:8257677-Cholic Acids,
pubmed-meshheading:8257677-Detergents,
pubmed-meshheading:8257677-Dimyristoylphosphatidylcholine,
pubmed-meshheading:8257677-G(M3) Ganglioside,
pubmed-meshheading:8257677-Lipid Bilayers,
pubmed-meshheading:8257677-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8257677-Molecular Conformation,
pubmed-meshheading:8257677-Molecular Structure,
pubmed-meshheading:8257677-N-Acetylneuraminic Acid,
pubmed-meshheading:8257677-Physicochemical Phenomena,
pubmed-meshheading:8257677-Sialic Acids,
pubmed-meshheading:8257677-Wheat Germ Agglutinins
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Structure and dynamics of the sialic acid moiety of GM3-ganglioside at the surface of a magnetically oriented membrane.
|
|
pubmed:affiliation |
Department of Chemistry, Yale University, New Haven, Connecticut 06511.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
