| pubmed-article:825679 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C0023443 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C0341628 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C0034789 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C1517806 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C0026249 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:825679 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:825679 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:825679 | pubmed:dateCreated | 1977-1-25 | lld:pubmed |
| pubmed-article:825679 | pubmed:abstractText | In four patients with hairy cell leukemia the expression of surface immunoglobulins and Fc-receptors on the leukemic cells as well as the stimulation of the leukemic cells by various T- and B-cell mitogens was studied. Using a combined cytochemical radioautographic method the tartrate resistant isoenzyme of the acid phosphatase and immunological markers could be demonstrated simultaneously on single cells. Surface immunoglobulins were detected by 125I-labelled (Fab')2-fragments of monospecific anti-heavy-chain-antibodies. In two patients only mu- and delta-determinants were found on the isoenzyme-positive hairy cells; in one patient 86% and in the other 44% of these cells carried both heavy chains on the same cell. Another patient showed both gamma- and delta-chains on the isoenzyme-positive cells and the fourth patient gamma-chains only. When the hairy cells of one patient were cultured in vitro in human serum-free medium for 14 days, mu- and delta-determinants were found just as on freshly isolated cells suggesting that hairy cells synthesize immunoglobulins. By indirect immunofluorescence the surface-Ig on hairy cells was shown to be capped very rapidly at room temperature. The concentrated surface-Ig was found over that cytoplasmic area where most of the ingested latex particles were located. In addition, using 125I-labelled aggregated human IgG (M.W. 5-15 X 10(6)), Fc-receptors were found on virtually all of the isoenzyme-containing hairy cells in all four patients. Furthermore, a distinct, low-degree stimulation of the isoenzyme-positive hairy cells could be demonstrated when the cells were cultured in the presence of pokeweed mitogen or Lima-bean lectin (B- and T-cell-mitogens), whereas no stimulation was observed by phytohemagglutinin and concanavalin A (T-cell-mitogens). In three patients studied, isoenzyme-positive hairy cells were negative with regard to rosette formation with sheep erythrocytes. | lld:pubmed |
| pubmed-article:825679 | pubmed:language | ger | lld:pubmed |
| pubmed-article:825679 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:825679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:825679 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:825679 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:825679 | pubmed:issn | 0023-2173 | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:WallerH DHD | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:RiethmüllerGG | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:RieberE PEP | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:LinkeR PRP | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:HeydenH WHW | lld:pubmed |
| pubmed-article:825679 | pubmed:author | pubmed-author:SaalJ GJG | lld:pubmed |
| pubmed-article:825679 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:825679 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:825679 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:825679 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:825679 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:825679 | pubmed:pagination | 1011-9 | lld:pubmed |
| pubmed-article:825679 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-H... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-P... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-L... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-A... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-M... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-I... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-L... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-I... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-I... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-I... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-B... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-B... | lld:pubmed |
| pubmed-article:825679 | pubmed:meshHeading | pubmed-meshheading:825679-R... | lld:pubmed |
| pubmed-article:825679 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:825679 | pubmed:articleTitle | [Hairy cell leukemia. Characterization of the leukemic cell: surface immunoglobulins, Fc-receptors and stimulation by mitogens (author's transl)]. | lld:pubmed |
| pubmed-article:825679 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:825679 | pubmed:publicationType | English Abstract | lld:pubmed |
