|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
1994-1-12
|
|
pubmed:abstractText |
Fragments of the alpha-toxin of Clostridium perfringens have been produced using genetic manipulation techniques. Antibody which cross-reacted with the alpha-toxin was induced after immunization with fragments representing the N- (Cpa1-249) and C-terminal (Cpa247-370) domains of the toxin. Smaller fragments of the alpha-toxin did not induce cross-reacting antibody. Anti-Cpa1-249 serum neutralized phospholipase C activity but not haemolytic activity of the toxin. Anti-Cpa247-370 serum neutralized both the phospholipase C and haemolytic activities. Only immunization with Cpa247-370 induced protection against the lethal effects of the toxin. Immunization with Cpa247-370 also provided protection in a mouse model against at least 10 LD100 doses of C. perfringens type A. This result confirms the essential role of this toxin in the pathogenesis of gas gangrene.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0264-410X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1253-8
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:8256506-Amino Acid Sequence,
pubmed-meshheading:8256506-Animals,
pubmed-meshheading:8256506-Antibodies,
pubmed-meshheading:8256506-Antibody Formation,
pubmed-meshheading:8256506-Bacterial Toxins,
pubmed-meshheading:8256506-Bacterial Vaccines,
pubmed-meshheading:8256506-Calcium-Binding Proteins,
pubmed-meshheading:8256506-Clostridium perfringens,
pubmed-meshheading:8256506-Disease Models, Animal,
pubmed-meshheading:8256506-Female,
pubmed-meshheading:8256506-Gas Gangrene,
pubmed-meshheading:8256506-Genetic Engineering,
pubmed-meshheading:8256506-Mice,
pubmed-meshheading:8256506-Mice, Inbred BALB C,
pubmed-meshheading:8256506-Molecular Sequence Data,
pubmed-meshheading:8256506-Peptide Fragments,
pubmed-meshheading:8256506-Type C Phospholipases,
pubmed-meshheading:8256506-Vaccines, Synthetic
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
A genetically engineered vaccine against the alpha-toxin of Clostridium perfringens protects mice against experimental gas gangrene.
|
|
pubmed:affiliation |
Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK.
|
|
pubmed:publicationType |
Journal Article
|
