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rdf:type |
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lifeskim:mentions |
umls-concept:C0007292,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0019704,
umls-concept:C0036126,
umls-concept:C0080194,
umls-concept:C0185117,
umls-concept:C0314603,
umls-concept:C0332161,
umls-concept:C1519878,
umls-concept:C1622204,
umls-concept:C1948027,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
1994-1-12
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pubmed:abstractText |
A peptide comprising residues glu293 to ser334 from the principal neutralization determinant (V3 loop) of the envelope of human immunodeficiency virus type 1 (HIV1 LAVBRU isolate) has been inserted within internal permissive sites of either LamB or MalE, two envelope proteins from Escherichia coli K12. The MalE hybrid protein (MalE133-V3 loop) was stably expressed in the periplasm of Escherichia coli K12, and the V3 loop peptide was detectable on the surface of the native protein by an anti-gp160 monoclonal antibody (mAb 110-A). The disulfide bridge between the two cysteines of the loop was formed. In contrast, genetic coupling to the outer membrane protein LamB did not allow the expression of a stable hybrid protein, and major proteolytic cleavage products of the LamB153-V3 loop were detected by mAb 110-A. The two plasmid-encoded hybrid genes were transferred to an aroA mutant of Salmonella typhimurium. Constitutive expression of the MalE133-V3 loop had no detectable effect on cell growth and on the survival in vivo of the recipient strain. The LamB153-V3 loop was not stably expressed in Salmonella, either in vitro or in vivo. Live recombinant salmonellas expressing MalE-V3 and LamB-V3 loop hybrids were used to immunize mice. The MalE-V3 loop hybrid induced anti-HIV1 envelope antibodies detectable by Western blot and ELISA, while the anti-HIV1 envelope antibodies induced by the LamB-V3 loop hybrid were only detectable by Western blot. In addition, purified MalE-V3 loop hybrid protein was able to stimulate in vitro and induce in vivo a V3 loop-specific T-cell proliferative response.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp160,
http://linkedlifedata.com/resource/pubmed/chemical/MalE protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Maltose-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Periplasmic Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Porins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/maltoporins,
http://linkedlifedata.com/resource/pubmed/chemical/maltose transport system, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1221-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8256503-AIDS Vaccines,
pubmed-meshheading:8256503-ATP-Binding Cassette Transporters,
pubmed-meshheading:8256503-Amino Acid Sequence,
pubmed-meshheading:8256503-Animals,
pubmed-meshheading:8256503-Antibody Formation,
pubmed-meshheading:8256503-Bacterial Outer Membrane Proteins,
pubmed-meshheading:8256503-Base Sequence,
pubmed-meshheading:8256503-Carrier Proteins,
pubmed-meshheading:8256503-Escherichia coli,
pubmed-meshheading:8256503-Escherichia coli Proteins,
pubmed-meshheading:8256503-Gene Products, env,
pubmed-meshheading:8256503-HIV Envelope Protein gp160,
pubmed-meshheading:8256503-HIV-1,
pubmed-meshheading:8256503-Lymphocyte Activation,
pubmed-meshheading:8256503-Maltose-Binding Proteins,
pubmed-meshheading:8256503-Mice,
pubmed-meshheading:8256503-Mice, Inbred BALB C,
pubmed-meshheading:8256503-Molecular Sequence Data,
pubmed-meshheading:8256503-Monosaccharide Transport Proteins,
pubmed-meshheading:8256503-Periplasmic Binding Proteins,
pubmed-meshheading:8256503-Porins,
pubmed-meshheading:8256503-Protein Precursors,
pubmed-meshheading:8256503-Receptors, Virus,
pubmed-meshheading:8256503-Recombinant Fusion Proteins,
pubmed-meshheading:8256503-Salmonella typhimurium,
pubmed-meshheading:8256503-Sensitivity and Specificity,
pubmed-meshheading:8256503-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
Expression and immunogenicity of the V3 loop from the envelope of human immunodeficiency virus type 1 in an attenuated aroA strain of Salmonella typhimurium upon genetic coupling to two Escherichia coli carrier proteins.
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pubmed:affiliation |
Programmation Moleculaire et Toxicologie Génétique (CNRS URA 1444), Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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