Linked Life Data

8256434

Source:http://linkedlifedata.com/resource/pubmed/id/8256434

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-1-13
pubmed:abstractText
An assay for assessing activation of the bovine alternative pathway of complement was developed. The assay focused on events on the surface of yeast. Yeast cells were incubated with EGTA-Mg2+ plasma, washed and the yeast-bound complement proteins eluted by 100 mM methylamine. Detection of eluted proteins was achieved by Western blot and ELISA. An ELISA for the quantification of the Bb fragment of factor B was chosen to measure activation of the alternative pathway of complement. Using this system, it was possible to demonstrate the kinetics of deposition of Bb on yeast incubated with plasma samples from individual cattle and to show differences between cattle. We were able to categorize cattle into 'fast or slow amplifiers' of the alternative pathway of complement. We suggest that this classification has implications for host protection against invading microorganisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A non-hemolytic assay for the activation of the alternative pathway of bovine complement.
pubmed:affiliation
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't