Linked Life Data

825609

Source:http://linkedlifedata.com/resource/pubmed/id/825609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1977-1-28
pubmed:abstractText
Pseudomonas aeruginosa PAC7 (a mutant deficient in diaminopimelate epimerase), excreted diaminopimelate (solely LL-isomer) after growth in a minimal medium plus lysine with succinate as carbon source. More diaminopimelate was excreted when bacteria were transferred at the end of the exponential phase of growth into fresh minimal medium without lysine but supplemented with pyrivate and additional (NH4)2SO4. The excreted LL-isomer was isolated from the culture filtrate by ion-exchange chromatography and purified by crystallization (1.7 g/9 1 culture). A diaminopimelate-requiring mutant of Bacillus megaterium NCIB7581 grew on LL- and/or meso-diaminopimelate but not on the DD-isomer. This mutant was used to isolate the DD-idomer from a mixture of synthetic LL- and DD-diaminopimelate. It was grown in a minimal medium containing glycerol as carbon source and LL- plus DD-diaminopimelate at a growth-limiting concentration (300 mg l-1); when growth stopped, the DD-diaminopimelate that remained in the culture was isolated and crystallized (1.0 g/II l culture).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-61
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Use of auxotrophic mutants to isolate LL- or DD-isomers of 2,6-diaminopimelic acid.
pubmed:publicationType
Journal Article