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rdf:type |
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lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0017890,
umls-concept:C0018787,
umls-concept:C0020663,
umls-concept:C0020792,
umls-concept:C0030956,
umls-concept:C0032005,
umls-concept:C0376315,
umls-concept:C1135183,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
4
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pubmed:dateCreated |
1994-1-11
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pubmed:abstractText |
Two chemical assays have been developed for identifying and quantifying peptides which either could be biologically active by virtue of their alpha-carboxamidation or could be substrates for peptidylglycine alpha-amidating mono-oxygenase. The first assay is specific for the alpha-carboxamide of peptides. Using bis[trifluoroacetoxy]iodobenzene, the alpha-carboxamide was converted via a Hoffman reaction into a primary amine, which was then quantified by ninhydrin. The second assay is specific for glycine at the carboxy-terminus of a peptide. Glycine at the carboxy-terminus was derivatized to form 2-thiohydantoin, which was then separated and quantified by reverse phase HPLC. These assays were used to detect peptides in HPLC-separated extracts of bovine hypothalamus, bovine anterior lobe pituitary and porcine heart which may be of biological interest.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0143-4179
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8255401-Acetylation,
pubmed-meshheading:8255401-Amides,
pubmed-meshheading:8255401-Amino Acid Sequence,
pubmed-meshheading:8255401-Animals,
pubmed-meshheading:8255401-Asparagine,
pubmed-meshheading:8255401-Cattle,
pubmed-meshheading:8255401-Chromatography, High Pressure Liquid,
pubmed-meshheading:8255401-Glutamine,
pubmed-meshheading:8255401-Glycine,
pubmed-meshheading:8255401-Hypothalamus,
pubmed-meshheading:8255401-Mass Spectrometry,
pubmed-meshheading:8255401-Molecular Sequence Data,
pubmed-meshheading:8255401-Myocardium,
pubmed-meshheading:8255401-Oxidation-Reduction,
pubmed-meshheading:8255401-Peptides,
pubmed-meshheading:8255401-Pituitary Gland, Anterior,
pubmed-meshheading:8255401-Swine,
pubmed-meshheading:8255401-Thiohydantoins
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pubmed:year |
1993
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pubmed:articleTitle |
Identification of alpha-carboxamidated and carboxy-terminal glycine forms of peptides in bovine hypothalamus, bovine pituitary and porcine heart extracts.
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pubmed:affiliation |
Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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