Linked Life Data

8254311

Source:http://linkedlifedata.com/resource/pubmed/id/8254311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1994-1-10
pubmed:abstractText
The possible functions of two recently described flagellar genes, fliU and fliV, have been examined. Introduction of gene fliC, encoding the bacterial flagellin protein, into a number of flagellin-deficient Salmonella and Escherichia coli strains failed to complement the mutations in these strains, and the FliC flagellin was accumulated in the bacterial cytoplasm. Complementation with fliU and fliV, which map downstream of fliC, restored motility to some of the mutants which became flagellated. After inactivation of either fliU or fliV, such complementation no longer occurred and the flagellin protein accumulated in the cytoplasm, which suggested that both genes are required for the secretion of flagellin and expression of motility. Expression of these genes from high copy number plasmids resulted in the synthesis of exceptionally long flagella and in detection of the FliV protein on polyacrylamide gels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:geneSymbol
fliU, fliV
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2415-22
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
fliU and fliV: two flagellar genes essential for biosynthesis of Salmonella and Escherichia coli flagella.
pubmed:affiliation
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article