| pubmed-article:8253960 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C0008151 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C1550252 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C1516634 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:8253960 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:8253960 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:8253960 | pubmed:dateCreated | 1994-1-10 | lld:pubmed |
| pubmed-article:8253960 | pubmed:abstractText | A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs (CP24-CP27), uracil-N-glycosylase (AmpErase), and a microtiter format for amplicon capture and detection. Culture with McCoy cells in duplicate 1-dram (3.697-ml) vials with fluorescent immunostaining was the reference system. Endocervical swab samples from 945 women provided 74 culture-positive specimens, of which PCR detected 71. The initial PCR result was positive for 12 additional specimens. Arbitration of the PCR-positive, culture-negative samples by PCR with major outer membrane protein primers, duplicate culture, elementary body direct fluorescent-antibody staining, and DNA extraction PCR showed that all 12 samples were positive for chlamydia, raising the number of truly positive samples from 74 to 86. After arbitration the true sensitivities of PCR and culture were 96.5 and 86%, respectively (P = 0.02). Specificities for both were 100%. For PCR, the positive and negative predictive values were 100 and 99.7%, respectively. Total test efficiency was 99.7%. A high-test-volume (121 samples) timing study with all items included in the College of American Pathologists work load method indicated that this PCR format took approximately 3 min per sample. Because of the high sensitivity, specificity, and improved ease of handling, we found PCR to be a good alternative to culture for detection of C. trachomatis. | lld:pubmed |
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| pubmed-article:8253960 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8253960 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8253960 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8253960 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:8253960 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:8253960 | pubmed:author | pubmed-author:JungkindD LDL | lld:pubmed |
| pubmed-article:8253960 | pubmed:author | pubmed-author:SilvermanN... | lld:pubmed |
| pubmed-article:8253960 | pubmed:author | pubmed-author:BassC ACA | lld:pubmed |
| pubmed-article:8253960 | pubmed:author | pubmed-author:BondiJ MJM | lld:pubmed |
| pubmed-article:8253960 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8253960 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:8253960 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8253960 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8253960 | pubmed:pagination | 2648-53 | lld:pubmed |
| pubmed-article:8253960 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8253960 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8253960 | pubmed:articleTitle | Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. | lld:pubmed |
| pubmed-article:8253960 | pubmed:affiliation | Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107. | lld:pubmed |
| pubmed-article:8253960 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8253960 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8253960 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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