Linked Life Data

8253960

Source:http://linkedlifedata.com/resource/pubmed/id/8253960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1994-1-10
pubmed:abstractText
A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs (CP24-CP27), uracil-N-glycosylase (AmpErase), and a microtiter format for amplicon capture and detection. Culture with McCoy cells in duplicate 1-dram (3.697-ml) vials with fluorescent immunostaining was the reference system. Endocervical swab samples from 945 women provided 74 culture-positive specimens, of which PCR detected 71. The initial PCR result was positive for 12 additional specimens. Arbitration of the PCR-positive, culture-negative samples by PCR with major outer membrane protein primers, duplicate culture, elementary body direct fluorescent-antibody staining, and DNA extraction PCR showed that all 12 samples were positive for chlamydia, raising the number of truly positive samples from 74 to 86. After arbitration the true sensitivities of PCR and culture were 96.5 and 86%, respectively (P = 0.02). Specificities for both were 100%. For PCR, the positive and negative predictive values were 100 and 99.7%, respectively. Total test efficiency was 99.7%. A high-test-volume (121 samples) timing study with all items included in the College of American Pathologists work load method indicated that this PCR format took approximately 3 min per sample. Because of the high sensitivity, specificity, and improved ease of handling, we found PCR to be a good alternative to culture for detection of C. trachomatis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1400986, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1452654, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1500521, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1572969, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1597199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1681215, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1774342, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1993765, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-1997528, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-2071122, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-2073896, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-2179265, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-2199495, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-2229379, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-3191065, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-3282465, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-3284899, http://linkedlifedata.com/resource/pubmed/commentcorrection/8253960-8501220
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2648-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens.
pubmed:affiliation
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't