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rdf:type |
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lifeskim:mentions |
umls-concept:C0007054,
umls-concept:C0011777,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0019602,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0039654,
umls-concept:C0185117,
umls-concept:C0205195,
umls-concept:C2349975,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1994-1-4
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pubmed:databankReference |
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pubmed:abstractText |
We previously reported that the induction of L-histidine decarboxylase (HDC) in mouse mastocytoma cells was synergistically potentiated with a combination of dexamethasone and 12-O-tetradecanoylphorbol-13-acetate (TPA) [Biochim. Biophys. Acta, 1133, 172-178 (1992)]. To clarify the molecular mechanism of this synergistic action on HDC expression, we have isolated genomic DNA clone (MGH5), including 5'-flanking region of the mouse HDC gene. The transcription start site and the nucleotide sequences of the promoter regions were determined. We found that this clone contains a TATA-like box and a GC-box in the promoter region, and several putative binding sites for regulatory proteins in the 5'-flanking region. With mastocytoma cells transiently transfected with 5' deletion constructs of HDC-CAT fusion gene, it was found that the sequence from -267 to -43 is essential for the regulatory elements(s) involved in the increased transcription of the HDC gene with dexamethasone and TPA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
196
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1113-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8250869-Amino Acid Sequence,
pubmed-meshheading:8250869-Animals,
pubmed-meshheading:8250869-Base Sequence,
pubmed-meshheading:8250869-Cloning, Molecular,
pubmed-meshheading:8250869-DNA, Neoplasm,
pubmed-meshheading:8250869-DNA Primers,
pubmed-meshheading:8250869-Dexamethasone,
pubmed-meshheading:8250869-Drug Synergism,
pubmed-meshheading:8250869-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8250869-Genomic Library,
pubmed-meshheading:8250869-Histidine Decarboxylase,
pubmed-meshheading:8250869-Lung,
pubmed-meshheading:8250869-Mast-Cell Sarcoma,
pubmed-meshheading:8250869-Mice,
pubmed-meshheading:8250869-Molecular Sequence Data,
pubmed-meshheading:8250869-Polymerase Chain Reaction,
pubmed-meshheading:8250869-Promoter Regions, Genetic,
pubmed-meshheading:8250869-Restriction Mapping,
pubmed-meshheading:8250869-Stomach,
pubmed-meshheading:8250869-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8250869-Transcription, Genetic,
pubmed-meshheading:8250869-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Enhanced expression of the mouse L-histidine decarboxylase gene with a combination of dexamethasone and 12-O-tetradecanoylphorbol-13-acetate.
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pubmed:affiliation |
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article
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