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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-1-5
|
| pubmed:abstractText |
In producing mutant mice by gene-targeting and gene-trapping in embryonic stem (ES) cells, the efficient colonization of the mutant ES cells into germline is still a critical matter. We have established a new line of ES cells, TT2, from an F1 embryo between a C57BL/6 female and a CBA male. When the TT2 cells were injected into blastocysts, the colonization into each tissue was very low. However, when injected into eight-cell embryos, the cells segregated inside the blastomeres, localized in an inner cell mass of blastocysts developed 1 day later, and colonized efficiently in each tissue of the pups. The pups were disproportionately male, about half of which were composed of TT2-derived cells primarily; in more than 70% of the males, TT2-derived cells were dominant, accounting for over half of the total cells. When these males were mated, they exclusively yielded TT2-derived offspring. The germline-differentiating potency was stable during 3 weeks of culture. Twenty-one of 24 mutant clones independently isolated yielded germline chimeras, and 19 clones yielded them in a rate comparable to that of the parent cells. Thus, TT2 cells can serve as a valuable vehicle for the production of mutant mice.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
214
|
| pubmed:geneSymbol |
fyn,
p53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
70-6
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8250257-Animals,
pubmed-meshheading:8250257-Blastocyst,
pubmed-meshheading:8250257-Blotting, Southern,
pubmed-meshheading:8250257-Cell Differentiation,
pubmed-meshheading:8250257-Cell Line,
pubmed-meshheading:8250257-Chimera,
pubmed-meshheading:8250257-Chromosome Mapping,
pubmed-meshheading:8250257-Culture Techniques,
pubmed-meshheading:8250257-DNA,
pubmed-meshheading:8250257-Embryo, Mammalian,
pubmed-meshheading:8250257-Female,
pubmed-meshheading:8250257-Genetic Techniques,
pubmed-meshheading:8250257-Germ Cells,
pubmed-meshheading:8250257-Male,
pubmed-meshheading:8250257-Mice,
pubmed-meshheading:8250257-Mice, Inbred BALB C,
pubmed-meshheading:8250257-Mice, Inbred C57BL,
pubmed-meshheading:8250257-Mice, Inbred CBA,
pubmed-meshheading:8250257-Mice, Inbred Strains,
pubmed-meshheading:8250257-Mice, Mutant Strains,
pubmed-meshheading:8250257-Mutation,
pubmed-meshheading:8250257-Polymerase Chain Reaction,
pubmed-meshheading:8250257-Sex Characteristics,
pubmed-meshheading:8250257-Sex Ratio,
pubmed-meshheading:8250257-Stem Cells
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A novel ES cell line, TT2, with high germline-differentiating potency.
|
| pubmed:affiliation |
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|