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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1994-1-4
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pubmed:abstractText |
The characteristic histopathology and major histocompatibility complex associations in juvenile rheumatoid arthritis suggest an oligoclonal antigen-specific T-cell population may be critical to pathogenesis. To test this, we analyzed the T-cell repertoire of a polyarticular HLA-DR4+ juvenile rheumatoid arthritis patient with an aggressive form of disease that required arthrocentesis of the knee joints and early replacement of both hip joints. A comparison of T-cell-receptor beta chain variable region (V beta) gene expression in peripheral blood and synovial fluid performed by semiquantitation of cDNA samples amplified by the PCR revealed overexpression of the T-cell-receptor V beta 14 gene family. To determine the nature of V beta 14 overexpression, we sequenced randomly cloned amplification products derived from two synovial fluid, two synovial tissue, and three peripheral blood samples by using a V beta 14/beta chain constant region primer pair. Sequence data showed that the T-cell response in the synovia was oligoclonal. Of four clones found, one was present in all joints examined and persisted over time. This clone accounted for 67% and 74% of all V beta 14+ clones sequenced in two synovial fluid samples and 75% and 40% in two synovial tissue samples. This clone was also found at a lesser frequency in peripheral blood samples. Further studies provided evidence for the presence of oligoclonally expanded populations of T cells utilizing the V beta 14 T-cell receptor in 6 of 27 patients examined. In contrast to the remaining patients studied, 3 with a late onset polyarticular course who exhibited especially marked clonality were characterized by features typical of adult rheumatoid arthritis (IgM rheumatoid factor-positive and HLA-DR4+). These data suggest a role for V beta 14+ T cells in a group of juvenile rheumatoid arthritis patients.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1315049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1387614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1622409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1656449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1660155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1678776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1722335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1857971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-1953812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2025307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2194461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2455603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2479030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2548202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2805419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-2963340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-3485433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-3726548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-6213027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-644340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-6610692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-6967490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-762241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-8387894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248215-8506275
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11104-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8248215-Amino Acid Sequence,
pubmed-meshheading:8248215-Arthritis, Juvenile Rheumatoid,
pubmed-meshheading:8248215-Base Sequence,
pubmed-meshheading:8248215-Clone Cells,
pubmed-meshheading:8248215-Gene Expression,
pubmed-meshheading:8248215-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:8248215-Genes,
pubmed-meshheading:8248215-Humans,
pubmed-meshheading:8248215-Male,
pubmed-meshheading:8248215-Molecular Sequence Data,
pubmed-meshheading:8248215-Oligodeoxyribonucleotides,
pubmed-meshheading:8248215-RNA, Messenger,
pubmed-meshheading:8248215-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8248215-Sequence Alignment,
pubmed-meshheading:8248215-Sequence Homology, Amino Acid,
pubmed-meshheading:8248215-T-Lymphocytes,
pubmed-meshheading:8248215-Time Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Dominant T-cell-receptor beta chain variable region V beta 14+ clones in juvenile rheumatoid arthritis.
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pubmed:affiliation |
Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229-3039.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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