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rdf:type |
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lifeskim:mentions |
umls-concept:C0031715,
umls-concept:C0036720,
umls-concept:C0205360,
umls-concept:C0376315,
umls-concept:C0439855,
umls-concept:C0812215,
umls-concept:C1334474,
umls-concept:C1515877,
umls-concept:C1706586,
umls-concept:C1879547,
umls-concept:C2746015
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pubmed:issue |
23
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pubmed:dateCreated |
1994-1-4
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pubmed:abstractText |
Recombinant Mek1 and Raf-1 proteins produced in Sf9 cells undergo a tight association both in vivo and in vitro, which apparently does not depend on additional factors or the kinase activity of Mek1 or Raf-1. The complex can be disrupted by two polyclonal antibodies raised against Raf-1 peptides. Coinfection with Raf-1 activates Mek1 > 150-fold, and coinfection with Raf-1 and Mek1 activates Erk1 approximately 90-fold. The activation of Mek1 by Raf-1 involves only serine phosphorylation, which is directly proportional to the extent of Mek1 activation. Phosphopeptide maps suggest a single Raf-1 phosphorylation site on mek1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1281467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1310074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1322292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1322500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1326789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1330321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1381507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1382311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1411546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1504018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1518847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1628739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1716348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1717989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1849075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-1904317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-2415976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-2547163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-6955305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8327501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8380494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8381423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8381718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8385802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8388132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8389470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8390681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8248196-8440264
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Map2k1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoserine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10947-51
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8248196-Animals,
pubmed-meshheading:8248196-Enzyme Activation,
pubmed-meshheading:8248196-MAP Kinase Kinase 1,
pubmed-meshheading:8248196-Macromolecular Substances,
pubmed-meshheading:8248196-Mice,
pubmed-meshheading:8248196-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:8248196-Phosphorylation,
pubmed-meshheading:8248196-Phosphoserine,
pubmed-meshheading:8248196-Protein Binding,
pubmed-meshheading:8248196-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8248196-Protein-Tyrosine Kinases,
pubmed-meshheading:8248196-Proto-Oncogene Proteins,
pubmed-meshheading:8248196-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:8248196-Recombinant Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Raf-1 forms a stable complex with Mek1 and activates Mek1 by serine phosphorylation.
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pubmed:affiliation |
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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