Linked Life Data

8247031

Source:http://linkedlifedata.com/resource/pubmed/id/8247031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1994-1-6
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21956, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21957, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21958, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21959, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21960, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21961, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21962, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21963, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21964, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21965, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21966, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21967, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21968, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21969, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21970, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21971, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L21972
pubmed:abstractText
Structural analysis of the human immunoglobulin repertoire holds promise for determining the basis of variable region gene usage in response to a variety of auto and exogenous antigens. Here we report the nucleotide sequences of the heavy and light chain variable regions expressed by three human monoclonal antibodies specific for two clinically relevant bacterial pathogens, Bordetella pertussis and Haemophilus influenzae type b. The cell lines were derived by in vitro stimulation of lymphocytes from spleen or tonsillar tissue, respectively, and bind to different antigens from the two organisms. The single B. pertussis antibody is of the IgM lambda isotype and utilizes the single VH6 gene segment in combination with a V lambda 2 gene and demonstrates limited somatic mutation, yet is highly indicative of an antigen-driven immune response. One H. influenzae antibody is of the IgG2 lambda isotype and expresses a VH3 gene segment with a V lambda 1 gene, while the second cell line produces an IgG3 lambda antibody expressing a combination of VH2/V lambda 3. Both molecules show evidence of somatic mutation. The D gene segments of the heavy chains vary in length and display limited sequence homology with known germline D segments. As demonstrated previously, JH4 predominates (two JH4 and one JH3) and all three utilize the J lambda 3 gene segment. In addition, we have isolated and sequenced a number of germline VH2 gene segments in an attempt to better understand the nature of the VH2 germline repertoire. In addition to contributing to the understanding of the human antibody repertoire, such clinically relevant molecules may prove to be a source of passive immunotherapy for those at risk to developing disease.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1601-16
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Molecular characterization of human antibodies to bacterial antigens: utilization of the less frequently expressed VH2 and VH6 heavy chain variable region gene families.
pubmed:affiliation
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't