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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1994-1-3
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pubmed:abstractText |
L-type pyruvate kinase (L-PK) gene expression is modulated by hormonal and nutritional conditions. We have previously shown that the glucose/insulin response element (GlRE) of the L-PK gene is built around two noncanonical E boxes (element L4) that cooperate closely with a contiguous binding site (element L3). We present in this report the identification of proteins that interact with both elements. The L3 site binds hepatocyte nuclear factor 4 (HNF4)- and COUP/TF-related proteins. In fibroblasts, the overexpression of HNF4 transactivates the L-PK promoter. On the contrary, COUP/TF strongly inhibits the active promoter in hepatocytes. The L4 site binds the major late transcription factor (MLTF) in vitro and ex vivo; mutations that suppress this binding activity also inactivated the GlRE function. Mutations transforming one or two noncanonical E boxes of element L4 into consensus MLTF/USF binding sites strongly increase the affinity for MLTF/USF and do not impair the glucose responsiveness. However, merely the ability to bind MLTF/USF does not seem to be sufficient to confer a GlRE activity: those elements in which one E box has been destroyed and the other has been transformed into a consensus MLTF/USF sequence bind MLTF/USF efficiently but do not confer a high glucose responsiveness on the L-PK gene promoter. Consequently, the full activity of the L-PK GlRE seems to require the cooperation between two putative MLTF/USF binding sites located in the vicinity of an HNF4 binding site.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1312668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1315961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1406667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1406684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1549130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1589769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1618827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1639815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1734282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1748280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1848696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-1899293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2006410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2026584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2115126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2246264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2249772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2251503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2279702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2338243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2545675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2585483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-2739739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-3011791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-3396073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-3821727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-8094701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-8483904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246989-8509413
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Upstream Stimulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Usf1 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
L-PK
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7725-33
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8246989-Animals,
pubmed-meshheading:8246989-Base Sequence,
pubmed-meshheading:8246989-Binding Sites,
pubmed-meshheading:8246989-DNA,
pubmed-meshheading:8246989-DNA-Binding Proteins,
pubmed-meshheading:8246989-Glucose,
pubmed-meshheading:8246989-Hepatocyte Nuclear Factor 4,
pubmed-meshheading:8246989-Insulin,
pubmed-meshheading:8246989-Liver,
pubmed-meshheading:8246989-Male,
pubmed-meshheading:8246989-Molecular Sequence Data,
pubmed-meshheading:8246989-Phosphoproteins,
pubmed-meshheading:8246989-Promoter Regions, Genetic,
pubmed-meshheading:8246989-Pyruvate Kinase,
pubmed-meshheading:8246989-Rats,
pubmed-meshheading:8246989-Rats, Sprague-Dawley,
pubmed-meshheading:8246989-Transcription Factors,
pubmed-meshheading:8246989-Upstream Stimulatory Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Functional characterization of the L-type pyruvate kinase gene glucose response complex.
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pubmed:affiliation |
Laboratoire de Recherches en Génétique et Pathologie Moléculaire, Institut Cochin de Génétique Moléculaire, INSERM U-129, CHU Cochin, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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