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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1994-1-3
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pubmed:abstractText |
EWS/FLI-1 is a chimeric protein formed by a tumor-specific 11;22 translocation found in both Ewing's sarcoma and primitive neuroectodermal tumor of childhood. EWS/FLI-1 has been shown to be a potent transforming gene, suggesting that it plays an important role in the genesis of these human tumors. We now demonstrate that EWS/FLI-1 has the characteristics of an aberrant transcription factor. Subcellular fractionation experiments localized the EWS/FLI-1 protein to the nucleus of primitive neuroectodermal tumor cells. EWS/FLI-1 specifically bound in vitro an ets-2 consensus sequence similarly to normal FLI-1. When coupled to a GAL4 DNA-binding domain, the amino-terminal EWS/FLI-1 region was a much more potent transcriptional activator than the corresponding amino-terminal domain of FLI-1. Finally, EWS/FLI-1 efficiently transformed NIH 3T3 cells, but FLI-1 did not. These data suggest that EWS/FLI-1, functioning as a transcription factor, leads to a phenotype dramatically different from that of cells expressing FLI-1. EWS/FLI-1 could disrupt normal growth and differentiation either by more efficiently activating FLI-1 target genes or by inappropriately modulating genes normally not responsive to FLI-1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1311254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1339307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1423247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1445800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1522903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1592263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1876833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1901401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1967982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1967983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-1988149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2001592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2001839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2005881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2044959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2070421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2170850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2193231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2218500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2336378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2667136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2813360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-2827041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-3004699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-6088787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-7678780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8425553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8445738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8449942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8502483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8510758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8515819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8246959-8516324
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
EWS
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7393-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8246959-3T3 Cells,
pubmed-meshheading:8246959-Animals,
pubmed-meshheading:8246959-Base Sequence,
pubmed-meshheading:8246959-Binding Sites,
pubmed-meshheading:8246959-Cell Line,
pubmed-meshheading:8246959-Cell Nucleus,
pubmed-meshheading:8246959-Cell Transformation, Neoplastic,
pubmed-meshheading:8246959-Chromosomes, Human, Pair 11,
pubmed-meshheading:8246959-Chromosomes, Human, Pair 22,
pubmed-meshheading:8246959-Cloning, Molecular,
pubmed-meshheading:8246959-DNA, Neoplasm,
pubmed-meshheading:8246959-DNA-Binding Proteins,
pubmed-meshheading:8246959-HeLa Cells,
pubmed-meshheading:8246959-Humans,
pubmed-meshheading:8246959-Mice,
pubmed-meshheading:8246959-Molecular Sequence Data,
pubmed-meshheading:8246959-Oncogenes,
pubmed-meshheading:8246959-Proto-Oncogene Protein c-fli-1,
pubmed-meshheading:8246959-Proto-Oncogene Proteins,
pubmed-meshheading:8246959-Recombinant Fusion Proteins,
pubmed-meshheading:8246959-Sarcoma, Ewing,
pubmed-meshheading:8246959-Trans-Activators,
pubmed-meshheading:8246959-Translocation, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1.
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pubmed:affiliation |
Department of Pediatrics, Gwynne Hazen Cherry Memorial Laboratories, University of California, Los Angeles.
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