| pubmed-article:8245479 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0009782 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0035544 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0004359 | lld:lifeskim |
| pubmed-article:8245479 | lifeskim:mentions | umls-concept:C0205332 | lld:lifeskim |
| pubmed-article:8245479 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:8245479 | pubmed:dateCreated | 1994-1-6 | lld:pubmed |
| pubmed-article:8245479 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:abstractText | SLE and mixed connective tissue disease (MCTD) are characterized by the presence of high titers of autoantibodies against uridine-rich RNA-small nuclear ribonucleoprotein (snRNP) Ag. Because the presence of such snRNP-reactive autoantibodies has recently been shown to be associated with polymorphisms of HLA, this study was undertaken to determine whether snRNP-reactive T cells could be identified and characterized from patients. PBMC were stimulated with affinity-purified snRNP Ag and cloned by limiting dilution in the presence of rIL-2 and rIL-4, snRNP-reactive human T cell clones were generated from three patients and two healthy blood donors who possessed disease-associated HLA genotypes. The cell surface phenotype of clones determined by flow cytometry was CD3+, CD4+, CD45RO+, TCR V alpha beta+. TCR V beta analysis, performed using V beta-specific primers and polymerase chain reaction, revealed that the T cell lines generated were clonal; a limited number of TCR V beta genes were expressed among the clones tested. All clones tested by mAb blocking of Ag-induced proliferation were restricted by HLA-DR. Several T cell clones were identified that were specific for B'/B or D polypeptides. These results demonstrate that snRNP-reactive T cells can be isolated from SLE and MCTD patients in vitro, and that Ag-driven expansion of such T cells could play a role in the immunopathogenesis of these diseases in vivo. | lld:pubmed |
| pubmed-article:8245479 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8245479 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8245479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245479 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8245479 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8245479 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:CaldwellC WCW | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:HillD LDL | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:TakedaYY | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:KaneokaHH | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:SharpG CGC | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:LeeD RDR | lld:pubmed |
| pubmed-article:8245479 | pubmed:author | pubmed-author:HoffmanR WRW | lld:pubmed |
| pubmed-article:8245479 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8245479 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8245479 | pubmed:volume | 151 | lld:pubmed |
| pubmed-article:8245479 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8245479 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8245479 | pubmed:pagination | 6460-9 | lld:pubmed |
| pubmed-article:8245479 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:meshHeading | pubmed-meshheading:8245479-... | lld:pubmed |
| pubmed-article:8245479 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8245479 | pubmed:articleTitle | Human T cell clones reactive against U-small nuclear ribonucleoprotein autoantigens from connective tissue disease patients and healthy individuals. | lld:pubmed |
| pubmed-article:8245479 | pubmed:affiliation | Department of Internal Medicine, University of Missouri-Columbia School of Medicine. | lld:pubmed |
| pubmed-article:8245479 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8245479 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8245479 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8245479 | lld:pubmed |
