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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1994-1-6
|
| pubmed:databankReference | |
| pubmed:abstractText |
SLE and mixed connective tissue disease (MCTD) are characterized by the presence of high titers of autoantibodies against uridine-rich RNA-small nuclear ribonucleoprotein (snRNP) Ag. Because the presence of such snRNP-reactive autoantibodies has recently been shown to be associated with polymorphisms of HLA, this study was undertaken to determine whether snRNP-reactive T cells could be identified and characterized from patients. PBMC were stimulated with affinity-purified snRNP Ag and cloned by limiting dilution in the presence of rIL-2 and rIL-4, snRNP-reactive human T cell clones were generated from three patients and two healthy blood donors who possessed disease-associated HLA genotypes. The cell surface phenotype of clones determined by flow cytometry was CD3+, CD4+, CD45RO+, TCR V alpha beta+. TCR V beta analysis, performed using V beta-specific primers and polymerase chain reaction, revealed that the T cell lines generated were clonal; a limited number of TCR V beta genes were expressed among the clones tested. All clones tested by mAb blocking of Ag-induced proliferation were restricted by HLA-DR. Several T cell clones were identified that were specific for B'/B or D polypeptides. These results demonstrate that snRNP-reactive T cells can be isolated from SLE and MCTD patients in vitro, and that Ag-driven expansion of such T cells could play a role in the immunopathogenesis of these diseases in vivo.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, Small Nuclear
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
151
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6460-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8245479-Adult,
pubmed-meshheading:8245479-Amino Acid Sequence,
pubmed-meshheading:8245479-Antibodies, Monoclonal,
pubmed-meshheading:8245479-Antigen-Presenting Cells,
pubmed-meshheading:8245479-Base Sequence,
pubmed-meshheading:8245479-Cell Line,
pubmed-meshheading:8245479-Clone Cells,
pubmed-meshheading:8245479-Female,
pubmed-meshheading:8245479-HLA-DR Antigens,
pubmed-meshheading:8245479-Humans,
pubmed-meshheading:8245479-Lupus Erythematosus, Systemic,
pubmed-meshheading:8245479-Lymphocyte Activation,
pubmed-meshheading:8245479-Male,
pubmed-meshheading:8245479-Middle Aged,
pubmed-meshheading:8245479-Mixed Connective Tissue Disease,
pubmed-meshheading:8245479-Molecular Sequence Data,
pubmed-meshheading:8245479-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8245479-Ribonucleoproteins, Small Nuclear,
pubmed-meshheading:8245479-T-Lymphocytes
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Human T cell clones reactive against U-small nuclear ribonucleoprotein autoantigens from connective tissue disease patients and healthy individuals.
|
| pubmed:affiliation |
Department of Internal Medicine, University of Missouri-Columbia School of Medicine.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|