Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-12-30
pubmed:abstractText
High-performance liquid chromatography (HPLC) was used for the characterization of sophorolipids, one of the most important types of glycolipid biosurfactants. By using gradient elution with a water-acetonitrile mixture on a reversed-phase (C18) column and evaporative light-scattering detection, resolution of all the important individual sophorolipids present in fermentation products was achieved. In addition to HPLC, a combination of techniques involving selective production by fermentation of sophorolipids, chemical conversions of the products, separation methods and, for identification of lipidic chains of sophorolipids, gas chromatography and mass spectrometry was used. This led to the identification of almost all significant compounds observed in HPLC, including several previously unreported sophorolipids. As a result, a rapid method is now available for investigations of the influence of fermentation conditions on the nature and quantitative distribution of the sophorolipid products obtained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
648
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-49
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Identification and determination of individual sophorolipids in fermentation products by gradient elution high-performance liquid chromatography with evaporative light-scattering detection.
pubmed:affiliation
Institut Français du Pétrole, Rueil-Malmaison, France.
pubmed:publicationType
Journal Article