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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1993-12-28
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pubmed:abstractText |
Two cell lines, RR5.ET-1 and RR1.ET-1, that produce monoclonal antibodies specific for the carboxyl-terminal heptapeptide of endothelin-1 (ET-1) have been cloned and stabilized. An RIA was developed to facilitate the evaluation and characterization of these monoclonal antibodies. The affinity constant of each MAb for ET-1, as determined by Scatchard analysis, was 5.74 x 10(8) M-1 for RR5.ET-1 and 4.15 x 10(7) M-1 for RR1.ET-1. The antibodies reacted specifically with the carboxyl-terminus (ET15-21) and did not cross-react with the amino-terminal amino acids (ET1-16). As expected, the antibodies cross-reacted with endothelin-2 (ET-2) and endothelin-3 (ET-3), and to a lesser extent, with the closely related sarafotoxins. Both MAbs retained about 55% reactivity with the ET-1 terminal sequence of Asp-Ile-Ile-Trp (ET18-21) but had no reactivity with the ET sequence His-Leu-Asp-Ile-Ile-Trp-Val-Asn (ET16-23) nor with Big ET-1 (ET1-39). These data strongly suggest that the terminal four amino acids of ET-1 are included in the MAb binding site. More importantly, the terminal Trp21 must be free, not linked to Val22 to retain reactivity with either of the MAbs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0272-457X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
455-66
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:8244418-Amino Acid Sequence,
pubmed-meshheading:8244418-Animals,
pubmed-meshheading:8244418-Antibodies, Monoclonal,
pubmed-meshheading:8244418-Antibody Affinity,
pubmed-meshheading:8244418-Antibody Specificity,
pubmed-meshheading:8244418-Cross Reactions,
pubmed-meshheading:8244418-Endothelins,
pubmed-meshheading:8244418-Hybridomas,
pubmed-meshheading:8244418-Mice,
pubmed-meshheading:8244418-Mice, Inbred BALB C,
pubmed-meshheading:8244418-Molecular Sequence Data,
pubmed-meshheading:8244418-Peptide Fragments,
pubmed-meshheading:8244418-Radioimmunoassay
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pubmed:year |
1993
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pubmed:articleTitle |
Production and characterization of monoclonal antibodies to the carboxyl-terminal heptapeptide of endothelin-1.
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pubmed:affiliation |
Diagnostics Drug Discovery, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
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pubmed:publicationType |
Journal Article
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