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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1994-1-5
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pubmed:abstractText |
1. It was recently proposed that acetylcholinesterase (AChE), in addition to its esteratic activity, has proteolytic activity such that it may cleave the beta-amyloid precursor (beta-APP) within the beta-amyloid sequence. The purpose of this paper was to examine further whether AChE or butyrylcholinesterase (BuChE) had associated proteinase activity that was involved in the metabolism of beta-APP. 2. The ability of various preparations of AChE and BuChE to hydrolyze two synthetic fragments of beta-APP695 as model substrates containing the normal and aberrant cleavage sites was studied. 3. Digestion of these synthetic substrates with commercial preparations of Electrophorus electricus AChE indicated the presence of a trypsin-like proteolytic activity cleaving each peptide at the carboxy-terminal side of an internal lysine residue. 4. Purification of the trypsin-like proteinase activity by aminobenzamidine affinity chromatography yielded a preparation that was devoid of AChE activity but retained all of the proteinase activity. 5. Amino-terminal sequence analysis of this preparation showed that the first 13 amino acid residues were identical to beta-pancreatic trypsin. 6. These data indicate that the proteinase activity found in these commercial preparations of AChE is due to contamination with trypsin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholinesterase,
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid Precursor Protein Secretases,
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid beta-Protein Precursor,
http://linkedlifedata.com/resource/pubmed/chemical/Butyrylcholinesterase,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0272-4340
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
279-87
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8242691-Acetylcholinesterase,
pubmed-meshheading:8242691-Amino Acid Sequence,
pubmed-meshheading:8242691-Amyloid Precursor Protein Secretases,
pubmed-meshheading:8242691-Amyloid beta-Protein Precursor,
pubmed-meshheading:8242691-Animals,
pubmed-meshheading:8242691-Artifacts,
pubmed-meshheading:8242691-Butyrylcholinesterase,
pubmed-meshheading:8242691-Chromatography, Affinity,
pubmed-meshheading:8242691-Electrophorus,
pubmed-meshheading:8242691-Endopeptidases,
pubmed-meshheading:8242691-Molecular Sequence Data,
pubmed-meshheading:8242691-Peptide Fragments,
pubmed-meshheading:8242691-Sequence Alignment,
pubmed-meshheading:8242691-Trypsin
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pubmed:year |
1993
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pubmed:articleTitle |
Proteolysis at the secretase and amyloidogenic cleavage sites of the beta-amyloid precursor protein by acetylcholinesterase and butyrylcholinesterase using model peptide substrates.
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pubmed:affiliation |
Division of Pharmacology, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article,
Comparative Study
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