Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-12-30
|
| pubmed:abstractText |
Anemia in patients with chronic renal failure (CRF) is multifactorial, and while the majority will respond to the paternal administration of recombinant human erythropoietin (r-HuEPO), a role for coexistent plasma inhibitors and stimulators, such as burst-promoting activity (BPA), remains controversial. To evaluate the latter possibility, eight individuals with CRF on long-term hemodialysis, were studied before (mean hemoglobin 58.4 +/- 8.0 g/l and after 12 weeks of r-HuEPO therapy (mean hemoglobin 100.4 +/- 18.3 g/l). In vitro erythroid cultures using erythroid burst forming unit (BFU-E) and erythroid colony forming unit (CFU-E) assays were performed, plating 5 x 10(4) bone marrow mononuclear cells and comparing growth in heat-inactivated autologous serum with AB serum. Using Step III sheep erythropoietin (Connaught Laboratories, Willowdale, Ontario, Canada) (n = 4), mean BFU-E pre-therapy were 89.7 +/- 75.1, CFU-E were 418.5 +/- 150.6, whereas the corresponding figures in AB serum were 2.5 +/- 2.9 and 197 +/- 94.19, p = 0.1, p = 0.01, respectively. Similarly, with r-HuEPO (EPOCONN: Connaught Laboratories, Willowdale, Ontario, Canada) (n = 4), mean BFU-E were 145.25 +/- 103.3 in autologous serum and 31.0 +/- 56.75 in AB serum (p = 0.04). As controls, erythroid progenitors from two normal donors yielded 69 and 61 BFU-E colonies in autologous serum and 103 and 42 in AB serum; the corresponding CFU-E were 52 and 235 versus 136 and 137.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
1066-5099
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
428-34
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8241954-Adolescent,
pubmed-meshheading:8241954-Adult,
pubmed-meshheading:8241954-Cells, Cultured,
pubmed-meshheading:8241954-Colony-Forming Units Assay,
pubmed-meshheading:8241954-Erythropoiesis,
pubmed-meshheading:8241954-Erythropoietin,
pubmed-meshheading:8241954-Female,
pubmed-meshheading:8241954-Humans,
pubmed-meshheading:8241954-Kidney Failure, Chronic,
pubmed-meshheading:8241954-Male,
pubmed-meshheading:8241954-Middle Aged,
pubmed-meshheading:8241954-Renal Dialysis,
pubmed-meshheading:8241954-Time Factors
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Burst-promoting activity in the serum of patients with chronic renal failure undergoing long-term hemodialysis.
|
| pubmed:affiliation |
University of Cape Town Leukaemia Centre, Department of Haematology, South Africa.
|
| pubmed:publicationType |
Journal Article
|