Linked Life Data

8235614

Source:http://linkedlifedata.com/resource/pubmed/id/8235614

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5135
pubmed:dateCreated
1993-12-8
pubmed:abstractText
A system for stable transformation of Toxoplasma gondii tachyzoites was developed that exploited the susceptibility of Toxoplasma to chloramphenicol. Introduction of the chloramphenicol acetyltransferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection resulted in parasites stably expressing CAT. A construct incorporating the tandemly repeated gene, B1, targeted efficiently to its homologous chromosomal locus. Knockout of the single-copy gene, ROP1, was also successful. Stable transformation should permit the identification and analysis of Toxoplasma genes important in the interaction of this opportunistic parasite with its host.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
262
pubmed:geneSymbol
B1, ROP1, SAG1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
911-4
pubmed:dateRevised
2007-3-19
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker.
pubmed:affiliation
Department of Microbiology and Immunology, Stanford University School of Medicine 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't