Linked Life Data

8231805

Source:http://linkedlifedata.com/resource/pubmed/id/8231805

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-12-21
pubmed:databankReference
pubmed:abstractText
We report here the discovery of a novel bacterial gene (cycH) whose product is involved in the biogenesis of most of the cellular cytochromes c. The cycH gene was detected in the course of characterizing a cytochrome oxidase-deficient Bradyrhizobium japonicum Tn5 mutant (strain COX3) in which the transposon insertion disrupted cycH. All of the c-type cytochromes detectable in aerobically grown B. japonicum wild-type cells were absent in the COX3 mutant, with the exception of cytochrome c1. A secondary phenotypic effect was the spectroscopic absence of the aa3-type cytochrome c oxidase. The nucleotide sequence of the cloned wild-type cycH gene predicted a membrane-bound 369-amino-acid protein with an M(r) of 39727. Results from studies on its membrane topology suggested that approximately 110 N-terminal amino acids are involved in anchoring the protein in the membrane, whereas the remaining two-thirds of the protein are exposed to the periplasm. We postulate that the CycH protein plays an essential role in an as yet unidentified periplasmic step in the biogenesis of holocytochromes c, except that of cytochrome c1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:geneSymbol
ccl1, ccl2, cycH, cycV, cycW, cycX
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
729-40
pubmed:dateRevised
2008-6-14
pubmed:meshHeading
pubmed-meshheading:8231805-Aerobiosis, pubmed-meshheading:8231805-Amino Acid Sequence, pubmed-meshheading:8231805-Bacterial Proteins, pubmed-meshheading:8231805-Base Sequence, pubmed-meshheading:8231805-Cell Membrane, pubmed-meshheading:8231805-Cytochrome c Group, pubmed-meshheading:8231805-Electron Transport Complex IV, pubmed-meshheading:8231805-Genetic Complementation Test, pubmed-meshheading:8231805-Membrane Proteins, pubmed-meshheading:8231805-Models, Molecular, pubmed-meshheading:8231805-Molecular Sequence Data, pubmed-meshheading:8231805-Mutagenesis, Insertional, pubmed-meshheading:8231805-Open Reading Frames, pubmed-meshheading:8231805-Phenotype, pubmed-meshheading:8231805-Recombinant Fusion Proteins, pubmed-meshheading:8231805-Rhizobiaceae, pubmed-meshheading:8231805-Sequence Homology, Nucleic Acid
pubmed:year
1993
pubmed:articleTitle
Formation of several bacterial c-type cytochromes requires a novel membrane-anchored protein that faces the periplasm.
pubmed:affiliation
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't